MRI: Acquisition of a IM-Q-TOF Mass Spectrometer

核磁共振项目包括购买最先进的IM-Q-TOF光谱仪，这将使来自德克萨斯州A&amp；M大学商业（A&M Commerce）四个部门的教师合作进行尖端的生化研究。化学、生物和环境科学、农业、健康和人类表现系的研究项目和学生的培训将从该仪器中受益匪浅。该项目涉及七个多学科合作，以利用IM-Q-TOF仪器的独特功能。Angel博士和科学技术仪器技术员Richard Miller将管理IM-Q-TOF，并就仪器的操作和功能向其他合作者提供必要的培训。获得IM-Q-TOF质谱仪将使学生获得备受追捧的质谱培训和研究经验。这些都是蛋白质组学的基本工具；这是一个有希望扩大和推进我们对蛋白质功能和影响疾病发病因素的认识的研究领域。通过收购IM-Q-TOF，使研究项目成为可能，将有助于教师将研究与教育结合起来，并将提高学生的科学意识，为他们未来的科学/技术机会做好充分准备。知识价值：获得最先进的IM-Q-TOF质谱仪将在精心设计的研究项目的开发、实施和评估中提高教师和学生的知识。IM-Q-TOF将通过为所涉及的研究项目提供卓越的生物分析化学、蛋白质组学和大中型生物分子的结构研究，为广泛的研究提供独特的能力。PI将使用IM-Q-TOF研究特定领域，包括：研究金属肽复合物的自组装；金属肽配合物的结构表征；测定多肽的气相、金属结合亲和力；探索金属肽配合物的能量分辨气相反应性；测定多肽的溶液相、金属结合亲和和水解动力学；开发葡萄酒质量控制的Q-TOF方法。该项目还将支持本提案中详述的合作研究：锌、铜和铁金属肽配合物的研究；新型蛋白质组学试剂钯和铂水配合物的质谱研究干细胞中糖脂类的鉴定极限运动诱导代谢产物的测定阴离子和分子卟啉受体的设计与合成类黄酮与淀粉样肽和Tau蛋白片段的相互作用以及，分离蛇在捕猎猎物时使用的气味化学物质。更广泛的影响：先进的质谱技术是生物化学研究知识爆炸的重要组成部分。没有博士学位的人有很多机会。授予机构吸引，留住和研究生具有先进的仪器知识和经验，是重要的科学研究的一部分。来自德克萨斯州东北部18所两年制学院的学生和教师将有机会使用该仪器与大学教师和学生合作开展研究项目。这些大学的总入学人数超过7万人，其中2万人参加科学课程，超过23%的学生来自代表性不足的群体。移动商务提供的仪器培训和研究的性质也将充分有利于为快速发展的生物分析、蛋白质组学、生物工程和生化职业市场提供适当培训的人才。在这些快速发展的科学领域，对合格候选人和知识的巨大需求将部分由A&amp；M-Commerce的高素质教师进行的研究补充。

CBET-0821247AngelThe MRI project involves the acquisition of a state-of-the-art IM-Q-TOF spectrometer will enable a collaboration of faculty from four departments at Texas A&M University-Commerce (A&M Commerce) to conduct cutting-edge biochemical research. The research programs and the training of students from the chemistry, biology and environmental sciences, agriculture, and health and human performance departments will benefit tremendously from this instrument.The project involves seven multidisciplinary collaborations to utilize the unique capabilities of an IM-Q-TOF instrument. Dr. Angel and the Science and Technologies instrument technician, Richard Miller, will manage the IM-Q-TOF and provide necessary training to other collaborators on the operation and capabilities of the instrument. Acquisition of the IM-Q-TOF mass spectrometer will allow students to achieve highly sought-after mass spectrometry training and research experience. These are essential tools in proteomics; a research field that has promising potential for expanding and advancing our knowledge of protein functions and the factors that influence the onset of disease. The research projects made possible through acquisition of an IM-Q-TOF will help faculty integrate research with education and will increase the scientific awareness among the students to adequately prepare them for future scientific/technological opportunities.Intellectual Merit: The acquisition of a state-of-the-art IM-Q-TOF mass spectrometer will advance the knowledge of both faculty and students as carefully designed research projects are developed, conducted and evaluated. The IM-Q-TOF will provide a unique capability for enabling a wide range of research by offering exceptional qualities for bioanalytical chemistry, proteomics and structural studies of mid-tolarge size biomolecules for the research projects involved. The PI will research specific areas using the IM-Q-TOF including: investigating the self-assembly of metal-peptide complexes; characterizing the structures of metal-peptide complexes; measuring the gas-phase, metal-binding affinities of peptides; exploring the energy-resolved, gas-phase reactivity of metal-peptide complexes; determining the solution phase, metal-binding affinities and hydrolysis kinetics of peptides; developing Q-TOF methods for wine quality control. The PI will also support collaborative research detailed in this proposal: Studies of Zn, Cu and Fe Metal-Peptide Complexes; Mass Spectrometric Study of Palladium and Platinum Aqua Complexes as New Proteomics Reagents; Identification of Glycolipids in Stem Cells; Determining Metabolites Induced Under Extreme Exercise; Design and Synthesis of Porphyrin Receptors for Anions and Molecules; Flavonoid Interactions with Segments of Amyloid Beta Peptide and Tau Protein; and, Isolating Scent Chemicals Utilized by Snakes for Hunting Prey.Broader Impact: Advanced mass spectrometry techniques are a vital part of the explosion in knowledge in biochemical research. There are tremendous possibilities for a non-Ph.D. granting institution to attract, retain, and graduate students with sophisticated instrumentation knowledge as well the experience of being a part of significant scientific research. Students and faculty from eighteen 2-year colleges in Northeast Texas will have the opportunity of collaborating with university faculty and students on research projects using this instrument. The total enrollment of these colleges is over 70,000 with 20,000 of these involved in science courses, and over 23% of the students from underrepresented groups. The nature of the instrumental training and research provided by A&M-Commerce will also be fully conducive in providing appropriately trained personnel for the rapidly developing bioanalytical, proteomic, bioengineering and biochemical career markets. The huge requirement for qualified candidates and knowledge in these rapidly growing science fields will be partially supplemented by the research conducted by highly qualified faculty at A&M-Commerce.