Collaborative Research: Prochlorococcus and its contribution to new production in the Sargasso Sea

海洋原氯球菌广泛存在于营养贫乏的亚热带和热带海洋中，在某些地区可贡献高达82%的初级生产力。与大多数其他浮游植物不同的是，培养的原氯球菌分离株不能同化NO3-。然而，洛马斯的团队使用了流式细胞术和稳定同位素示踪剂来证明在马尾藻海直接被原氯球菌同化。为了支持这些发现，马蒂尼和他的同事们已经证明，居住在混合层中的原氯球菌细胞携带有NO2-和N03-同化的基因，并且这些基因在田间种群中具有功能和表达。综合结果表明，未培养的原氯球菌谱系能够同化NO2-并在许多海洋地区促进新的产量-但总体意义尚不清楚。该项目的总体假设是，细胞特有的N03同化速率是环境营养浓度和细胞代谢潜力(即编码NO2-和NO3-同化的基因的存在)的函数。这个项目的具体研究问题是：1)NO3--原氯球菌定量上重要的营养来源，以及原氯球菌是否有助于新的产量？2)氮底物(NH4+、尿素、NO2-和NO3-)的季节和垂直变化对原氯球菌基因组含量和氧化氮吸收速率的影响？为了回答这些问题，PIS将结合使用高灵敏度营养测量、Loma开发的用于量化特定分类组中氮同化的流式细胞仪分析、元基因组学和qPCR分析来确定与原氯球菌相关的亚硝酸盐(Nira)和硝酸还原酶(NARB)基因的存在。使用这些工具，他们将通过三个完整的季节周期和整个真光区对原氯球菌中N3同化和N3同化基因的分布进行量化。此外，这些直接测量将通过操纵性中胚层实验(相互移植和营养添加实验)来扩大，以明确测试其假说的各个方面。PIs希望实现对N3同化率的直接(氮物种浓度的变化)和间接控制(原氯球菌的基因组适应)的机械理解。该项目最令人兴奋的成果之一将是更全面地了解野外装配中原氯球菌的营养生态。私人投资机构选择在马尾藻海进行这项研究，是因为这个地点提供了大量必要的支持数据和后勤基础设施，而且他们已经表明原氯球菌能够在这个地区同化硝酸盐。更广泛的影响：这个项目将在许多层面上产生更广泛的影响。首先，由NO2-和NO3-同化促进的原氯球菌具有以前未知的广泛生产力的潜力，这对于理解原氯球菌的生物地理学及其在海洋碳和氮循环中的作用具有重要意义。其次，它将举例说明基因组进化、细胞生理学和环境变量如何相互作用来塑造细菌在海洋中的生物地球化学作用。这些指标将以一种易于纳入生物地球化学和生态系统模型的方式展示其结果，包括那些与应对气候驱动的变异性而引起的区域和全球生物过程变化有关的模型。这个项目将为研究生(加州大学学院的一个)和本科生提供研究机会。该项目还将建立一个国际联系的教育推广计划，为加利福尼亚州和百慕大的高中教师和学生提供海洋微生物生态及其与全球海洋过程和生物地球化学的关系的实践经验。

The cyanobacterium Prochlorococcus marinus is ubiquitous in the oligotrophic subtropical and tropical oceans and can contribute up to 82% of the primary productivity in certain regions. In contrast to most other phytoplankton, cultured Prochlorococcus isolates cannot assimilate NO3-. However, Lomas' group has used flow cytometry and stable isotope tracers to demonstrate direct NO3- assimilation by Prochlorococcus in the Sargasso Sea. In support of these findings, Martiny and colleagues have shown that Prochlorococcus cells residing in the mixed layer carry genes for NO2- and NO3- assimilation, and that these genes are functional and expressed in field populations. The combined results suggest that uncultured lineages of Prochlorococcus are capable of NO3- assimilation and can contribute to new production in many oceanic regions - but the overall significance is yet unknown.The overarching hypothesis of this project is that cell-specific NO3- assimilation rate is a function of both the ambient nutrient concentrations and the metabolic potential of the cell (i.e. presence of genes encoding for NO2- and NO3- assimilation). The specific research questions of this project are:1) Is NO3- a quantitatively important nutrient source for Prochlorococcus and does Prochlorococcus contribute to new production?2) What is the influence of seasonal and vertical variation in nitrogen substrates (NH4+, urea, NO2-, andNO3-) on the genome content of Prochlorococcus and oxidized nitrogen uptake rates?To answer these questions, PIs will use the combination of high-sensitivity nutrient measurements, a flow cytometric assay developed by Lomas to quantify nitrogen assimilation in specific taxonomic groups, and metagenomics and a qPCR assay to determine the occurrence of nitrite (nirA) and nitrate reductase (narB) genes associated with Prochlorococcus. Using these tools, they will quantify NO3- assimilation and the distribution of NO3- assimilation genes in Prochlorococcus through three full seasonal cycles and over the entire euphotic zone. In addition, these direct measurements will be augmented by manipulative mesocosm experiments (reciprocal transplant and nutrient addition experiments) to explicitly test aspects of their hypotheses. The PIs hope to achieve a mechanistic understanding of direct (variations in the concentration of nitrogen species) and indirect controls (genomic adaptation in Prochlorococcus) on NO3- assimilation rates. One of the most exciting outcomes from this project will be a more complete understanding of the nutritional ecology of Prochlorococcus in field assemblages. The PIs have selected to conduct this study in the Sargasso Sea, because of the wealth of necessary supporting data and logistical infrastructure that this site provides, and because they have already shown that Prochlorococcus is capable of nitrate assimilation in this region.Broader Impacts: This project will have broader impacts on many levels. First, the potential for previously unrecognized widespread productivity by Prochlorococcus fueled byNO2- and NO3- assimilation has significant implications for the understanding of the biogeography of Prochlorococcus and its role in oceanic carbon and nitrogen cycles. Secondly, it will exemplify how genome evolution, cell physiology, and environmental variables interact to shape the biogeochemical role of bacteria in the ocean. The PIs will present their results in a manner that can be easily incorporated in biogeochemical and ecosystem models, including those related to changes in regional and global biological processes in response to climate-driven variability. This project will provide research opportunities for graduate (one at UCI) and undergraduate students. This project will also establish an internationally linked education outreach program to provide high school teachers and students in California and Bermuda with hands-on experience in marine microbial ecology and its relationship to global ocean processes and biogeochemistry.