Electroactivated Peptides for Dynamic Functionalization

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。0932989 Gower生物传感/CBET计划的NSF奖项支持佛罗里达大学材料工程师劳里高尔和大卫诺顿教授的工作，以探索电活性肽的新生物淘选方法的可行性。噬菌体展示组合系统将用于筛选具有可逆结合特性的无机结合肽，使得电场可用于触发肽从电子材料表面的吸附和/或解吸。 然后，这些肽可以作为连接物来连接其他感兴趣的组分，例如生物传感器阵列的生物受体，或芯片实验室配置的纳米颗粒“货物”。 这里提出的实验将为未来的应用奠定基础，首先探索不同的电子构型，以确定哪种刺激模式可以提供最佳的可逆性，而不会对肽或附着的生物组分产生不利影响。 虽然“分子仿生学”方法已经被考虑用于多组分系统的开发，其中肽接头可以被筛选为对不同的无机材料具有选择性结合亲和力，但这里正在探索的系统将使这项技术达到一个全新的水平，因为它可以提供一种用于动态图案化多组分表面的方法。静态微图案化表面已经为生物技术竞技场带来了重大进步;对表面功能进行空间和时间控制的能力可能是生物纳米技术的下一个革命性进步。 对于下一代XYZ-微系统，希望利用微电子的小尺寸和高效率，结合生物系统提供的高灵敏度和选择性。 最大的挑战是将非电子元件（如生物受体）与电子转换元件集成在一起。 这里提出的工作不仅将提供有机-无机界面之间的联系，而且还将提供对设备本身做出响应的联系，以实现真正的智能系统。 人们可以设想在真实的时间内改变表面图案以用于细胞的接触引导，或者用于生物分子穿梭的轨道的定向聚合（例如在智能灰尘生物传感器中）。 电活化肽可能带来的另一个令人兴奋的进步是设备表面的自清洁能力。 这可以实现连续的生物传感，其中可以设计一个流通系统，该系统被触发以释放堵塞的受体，然后用自组装到失活表面上的新鲜受体进行替换。 因此，这些探索性研究将为一项全新的技术奠定基础，这可能导致从军事到私人医疗保健部门的商业化应用。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).0932989GowerThis NSF award by the Biosensing/CBET program supports work by Professors Laurie Gower and David Norton, Materials Engineers at the University of Florida, to explore the feasibility of a new biopanning approach for electroactive peptides. The phage display combinatorial system will be used to screen for inorganic-binding peptides that have reversible binding properties so that an electric field can be used to trigger the sorption and/or desorption of the peptides from an electronic material's surface. These peptides can then serve as linkers to attach other components of interest, such as bioreceptors for biosensor arrays, or nanoparticle "cargo" for lab-on-a-chip configurations. The experiments proposed here will lay the groundwork for such future applications, by first exploring different electronic configurations to determine which mode of stimulus can provide the best reversibility without adversely affecting the peptide or attached biocomponent. While the "molecular biomimetics" approach is already being considered for the development of multicomponent systems, where peptide linkers can be screened to have selective binding affinity to different inorganic materials, the system being explored here will bring this technology to a whole new level because it could provide a means for dynamically patterning a multicomponent surface. Static micropatterned surfaces have already brought significant advances to the biotechnology arena; the ability to provide both spatial and temporal control over a surface's functionality could be the next revolutionary advance for bionanotechnology. For the next generation of XYZ-microsystems, it is desirable to capitalize on the small size and high efficiency of microelectronics, in combination with the high sensitivity and selectivity provided by biological systems. The grand challenge is in integrating non-electronic components (such as bioreceptors) with the electronic transduction elements. The work proposed here will provide not only a linkage between the organic-inorganic interface, but a linkage that is responsive to the device itself, for truly smart systems that assemble on command. One could envision changing a surface pattern in real time for contact guidance of cells, or directed polymerization of tracks for biomolecular shuttles (e.g. in smart dust biosensors). Another exciting advance that could result from electroactivated peptides is the self-cleaning capability of device surfaces. This could enable continuous biosensing, where a flow-through system could be designed that is triggered to release clogged receptors, followed by replacement with fresh receptors which self-assemble onto the de-activated surface. Thus, these exploratory studies will lay the groundwork for a whole new technology, which could lead to commercializable applications ranging from the military to private health care sectors.