Renilla Transfected Stem Cells as Light Source for In-Situ Photodynamic Therapy of Cancer

“该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。Bossmann光动力疗法引起细胞凋亡（程序性细胞死亡）而不是坏死，是治疗许多癌症的非常有前途的方法。由于癌细胞对卟啉受体的过度表达，许多PDT试剂可以高选择性地递送至癌细胞。然而，由于人体的生物物理特征，较差的光穿透仍然是治疗肿瘤的最大障碍，肿瘤不位于光可及表面附近。PI建议在小鼠模型中使用海肾荧光素酶的生物发光，通过原位PDT治疗B16 F10皮下黑色素瘤。这些黑色素瘤细胞在静脉注射时形成深部肺转移病灶，因此也将测试这种内部形式的肿瘤模型。研究结果将有助于开发更好的治疗癌症，不愈合伤口和细菌感染的方法。虽然癌症通常被称为一种疾病，但它实际上是由100多种不同的疾病组成的。根据美国癌症协会的说法，这些疾病的特征是不受控制的生长和异常细胞的扩散。癌症可以发生在许多部位，并根据其起源器官而表现不同。这种新的方法将结合联合收割机的处理方法，从干细胞生物学，光化学生物技术和物理无机化学。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."0933701BossmannPhotodynamic Therapy causing apoptosis (programmed cell death) rather than necrosis, is a very promising approach for the treatment of numerous cancers. Many PDT-agents can be delivered to cancer cells with high selectivity due to the overexpression of porphyrin-receptors by the cancer cells. However, due to the biophysical features of the human body, poor light penetration remains the biggest obstacle for the treatment of tumors, which are not located near a light accessible surface. The PI proposes to use the bioluminescence from Renilla luciferase in a mouse model to treat B16F10 subcutaneous melanomas with in-situ PDT. These melanoma cells form deep pulmonary metastatic lesions when given by I.V. injection, so this internal form of the tumor model will also be tested. The results will help to develop better treatments for cancer, non-healing wounds and bacterial infections. Although cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. According to the American Cancer Society, These diseases are characterized by uncontrolled growth and spread of abnormal cells. Cancer can arise in many sites and behave differently depending on its organ of origin. This new approach will combine treatment methods from stem cell biology, photochemical biotechnology and physical inorganic chemistry.