CAREER: Biosensors Based on Electrogenerated Chemiluminescence

在这个由化学学部化学测量和成像项目和EPSCoR办公室资助的职业奖中，来自南密西西比大学的苗教授和他的学生将进行基于电致化学发光（ECL）的生物传感器的研究。重点将是同时多路ECL检测和纳米材料样品基质中生物分子的定量。所提出的检测方案可能允许同时测量3-5个分析物，而不需要靶扩增或使用酶。通过使用不同类型的ECL标签（疏水、亲水性和量子点），ECL检测可以在有机或水溶液中进行，也可以在固态形式中进行。循环伏安法、ECL与扫描电化学显微镜相结合、电子顺磁共振波谱、电化学和ECL数字模拟等工具将用于实现这些目标。此外，还将研究和制备用于高性能ECL检测的透明电极的纳米厚度Au膜或Au纳米颗粒（NPs）涂层。最后，研究了光化学、电化学和ECL行为，并探索了模板化的微纳米级半导体金属硫族化物薄膜和NPs的传感应用。ECL生物传感器可以应用于临床诊断、法医化学、环境调查、药物研究，甚至在高炸药和生物战剂的敏感探测中——这是全球安全中的一个相关问题。苗教授的研究将与本科和研究生教育相结合。在此背景下，将与电化学仪器制造商一起开发一种廉价、易于操作、适应各种教学和学习环境的分析ECL实验室。此外，苗教授将与USM的同事合作，将仪器分析、无机化学和物理化学实验整合到一个本科高级化学实验室中。综合实验室的实施将成为该地区其他化学系的典范，并为USM和地区社区学院之间建立更牢固的关系提供机会。

In this Career award, funded by the Chemical Measurement and Imaging program of the Division of Chemistry and the EPSCoR office, Professor Miao from the University of Southern Mississippi and his students will conduct research on biosensors based on electrogenerated chemiluminescence (ECL). The focus will be on simultaneous multiplexing ECL detection and quantification of biomolecules in a sample matrix with nanomaterials. The proposed detection scheme may allow for simultaneous measurements of 3-5 analytes without the need for target amplification or the use of enzymes. By using different types of ECL labels (hydrophobic, hydrophilic, and quantum dots), ECL detection can be performed in either organic or aqueous solutions, or in a solid-state format. Tools such as cyclic voltammetry, ECL coupled with scanning electrochemical microscopy, electron paramagnetic resonance spectroscopy, and electrochemical and ECL digital simulations will be used to achieve these objectives. Furthermore, investigation and preparation of nano-thickness Au film or Au nanoparticles (NPs) coatings for transparent electrodes for high performance ECL detection will be carried out. Finally, investigations of the photochemical, electrochemical, and ECL behavior as well as an exploration of sensing applications of templated micro- to nano-scale semiconductive metal chalcogenide films and NPs will be conducted.ECL biosensors may find applications in clinical diagnostics, forensic chemistry, environmental investigations, pharmaceutical studies, and even in sensitive detection of high explosives and biological warfare agents - which is a pertinent issue in global security.Professor Miao's research will be integrated with undergraduate and graduate education. In this context, an analytical ECL lab that is inexpensive, easy to operate, and adaptable to various levels of teaching and learning environments will be developed with an electrochemical instrument manufacture. In addition, in collaboration with colleagues from USM, Professor Miao will integrate instrumental analysis and inorganic as well as physical chemistry experiments into one senior undergraduate chemistry lab. The implementation of an integrative lab will serve as a model for other chemistry departments in the region and provide the opportunity to forge more robust relationships between USM and regional community colleges.