CAREER: Field-free Transmission Geometry Atmospheric Pressure Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

韦恩州立大学（WSU）的Sarah Trimpin教授获得了化学测量和成像项目的CAREER奖，致力于开发、研究和采用新的质谱（MS）电离机制。国际科学与工程办公室的共同资助使这项工作得以扩展，将强大的质谱工具与柏林联邦材料研究与测试研究所（BAM）的Steffen Weidner博士团队的分离专业知识结合起来。质谱的最新进展使其成为表征包括生物和合成聚合物在内的广泛系统的至关重要的工具。Trimpin博士的工作是通过增强表征系统（例如细胞膜）中生物和物理过程的能力来扩大可接近问题的范围，这些系统的溶解度有限，阻碍了现有技术的研究。长期目标是开发不使用溶剂的质谱方法，提供细胞水平的分子采样，并实现完整的组织分析。Trimpin博士对基质辅助激光解吸/电离（MALDI）的创新修改首次提供了混合离子迁移谱（IMS） MS与高质量分析物（例如蛋白质）的高空间分辨率分析的耦合。该方法产生具有多个电荷的离子，从而可以利用电子转移离解（ETD）碎片，这是一种强大的表征方法，以前只能使用电喷雾电离（ESI）。Trimpin博士的团队寻求提高对这种新的MS电离方法的理解，这反过来应该加强对MALDI和ESI电离过程的理解。这项工作将在整个化学和生物学的研究领域产生广泛的影响。它可以提供信息，帮助我们了解细胞的功能和塑料的材料特性。Trimpin教授致力于跨学科教学和培训，以促进科学学习，为华盛顿州立大学的种族和性别多元化学生群体做好准备，为21世纪技术驱动的工作场所做好准备，从而增强了这项工作的影响。在她的研究和教学活动中，Trimpin教授通过与高中和大学女生的互动，鼓励女性从事科学事业，这是她的浓厚兴趣。她还在华盛顿州立大学建立了一个“妇女与科学”支持小组。

With this CAREER award from the Chemical Measurement and Imaging Program, Professor Sarah Trimpin at Wayne State University (WSU) seeks to develop, study, and employ new ionization mechanisms for mass spectrometry (MS). Co-funding from the Office of International Science and Engineering enables extension of the work to pair powerful mass spectrometric tools with separations expertise in the group of Dr. Steffen Weidner in the Federal Institute for Materials Research and Testing (BAM) - Berlin.Recent advances in MS have made it a critically important tool for characterization of a wide range of systems including bio- and synthetic polymers. Dr. Trimpin's work is expanding the range of problems approachable by enhancing capabilities for characterizing biological and physical processes in systems (e.g. cell membranes) where limited solubility impedes study with existing technology. The long-term goal is to develop MS methods that operate without the use of solvents, providing for molecular sampling at the cellular level and enabling complete tissue analyses. Dr. Trimpin's innovative modifications to matrix-assisted laser desorption/ionization (MALDI) offer the first coupling of hybrid ion mobility spectrometry (IMS) MS with high spatial resolution analysis of high-mass analytes (e.g. proteins). The approach produces ions with multiple charges, enabling exploitation of electron transfer dissociation (ETD) fragmentation, a powerful characterization method previously usable only with electrospray ionization (ESI). Dr. Trimpin's group seeks improved understanding of this new MS ionization method, which in turn should enhance understanding of MALDI and ESI ionization processes. The work will have broad impact in research areas throughout chemistry and biology. It can provide information that will help us understand the functioning of cells and the materials properties of plastics.Impact of this work is enhanced by Prof. Trimpin's dedication to interdisciplinary teaching and training in promotion of scientific learning for preparation of the ethnic- and gender-diverse student body at WSU for the technology-driven 21st century workplace. In concert with her research and teaching activities, Prof. Trimpin pursues her strong interest in encouraging women to follow scientific careers by interacting with high school and university female students. She is also developing a "Women and Science" support group at WSU.