MRI-R2: Acquisition of Biophysical Instruments for Interdisciplinary Faculty and Student Research

该奖项是根据2009年美国复苏和再投资法案资助的（公法111-5）。有了这个来自主要研究和仪器（MRI）计划的奖项，库马尔·辛尼亚教授和他的同事大卫E本森和艾米·威尔斯特曼从卡尔文学院，和布拉德沃拉尔从大谷州立大学将获得原子力显微镜（AFM），等温滴定量热仪（ITC），和差示扫描量热仪（DSC）。 该仪器将用于一系列化学和生物学研究，包括：G-四链体DNA与胰岛素和拓扑异构酶I之间的结合相互作用，控制蛋白质附着和纳米颗粒表面稳定性的热力学参数，以及参与透明相关formin调节的蛋白质相互作用。 原子力显微镜、等温滴定量热法和差示扫描量热法用于研究蛋白质-蛋白质和蛋白质-配体复合物从单分子到整体系综的结合。单分子相互作用将使用原子力显微镜（AFM）进行研究，而批量合奏的特点将使用等温滴定量热法（ITC）和差示扫描量热法（DSC）的互补技术进行。 ITC和DSC提供了关于蛋白质-蛋白质和蛋白质-配体相互作用的稳定性和结合的关键信息，并将有助于解释从AFM研究中估计的单分子动力学和热力学性质。 这些仪器将共同扩大研究和培训的范围，为卡尔文学院和大峡谷州立大学（GVSU）的教师和学生提供必要的生物分析能力。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research and Instrumentation (MRI) program, Professor Kumar Sinniah and his colleagues David E Benson and Amy Wilstermann from Calvin College, and Brad Wallar from Grand Valley State University will acquire an atomic force microscope (AFM), isothermal titration calorimeter (ITC), and differential scanning calorimeter (DSC). The instruments will be used in an array of chemical and biological investigations including: the binding interaction between G-quadruplex DNA with insulin and with topoisomerase I, the thermodynamic parameters that control protein attachment and stability on nanoparticle surfaces, and the protein interactions involved in the regulation of diaphanous related formins. Atomic force microscopy, isothermal titration calorimetry, and differential scanning calorimetry are used to study the binding of protein-protein and protein-ligand complexes from single molecule to bulk ensemble methods. The single molecule interactions will be studied using the atomic force microscope (AFM), while the bulk ensemble characterizations will be carried out using the complementary techniques of isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC). The ITC and DSC provide critical information regarding the stability and binding of protein-protein and protein-ligand interactions and will aid in the interpretation of single molecule kinetic and thermodynamic properties estimated from the AFM studies. Together these instruments will expand the scope of research and training possible, providing essential bioanalytical capabilities to faculty and students at Calvin College and Grand Valley State University (GVSU).