MRI-R2: Acquisition of a liquid chromatograph-mass spectrometer for biogeochemistry and paleoclimate studies at the Large Lakes Observatory

“这项奖励是根据2009年美国复苏和再投资法案（公法111-5）资助的。”π吗?申请资金购买最新的液相色谱仪？质谱仪（LC/MS）具体来说，我们需要一个带有电喷雾电离（ESI）、大气压化学电离（APCI）源的三重四极杆LC/MS系统，从而可以表征广泛的可溶性分子，包括提取的挥发性不足以适用气相色谱的脂质。该仪器的收购将允许表征广泛不同结构的分子，同时保持维护要求在我们目前的基础设施和技术支持人员支持的水平上。正在进行的和未来的项目包括脂质分析，用于开发和应用古环境代用物，水柱颗粒和溶解有机质的研究，完整极性脂质的研究，以及进一步发展黑碳测量。更广泛的影响：LC/MS三重四元系统的收购将为研究不适合GC/MS分析的化合物开辟新的途径。这项研究的意义可能是深远的，就黑碳的环境问题而言。教授本科生和研究生的研究是两个PI的真正优势。PI优先强调实践经验，包括研究生和本科生在三门不同课程上的研究经验。文本中弥漫着一种态度，即仪器不应该被用作“黑匣子”，而是作为一个实践学习的机会，这是该提案的一个重要优点。这方面符合国家科学基金会促进研究和教育一体化的主要战略之一。

"This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)."The PI?s request funding to acquire an up-to-date liquid chromatograph ? mass spectrometer (LC/MS). Specifically, we request a triple quadrupole LC/MS system with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) sources, thus allowing characterization of a wide range of soluble molecules, including extracted lipids that are not volatile enough to be GC-amenable. The acquisition of this instrument will allow characterization of molecules of widely varying structures, while keeping maintenance requirements at a level supportable with our current infrastructure and technical support staff. Ongoing and future projects include analysis of lipids for development and application of paleoenvironmental proxies, studies of water column particulate and dissolved organic matter, studies of intact polar lipids, and further development of black carbon measurements.Broader Impacts: The acquisition of the LC/MS triple quad system will open up new avenues of research into compounds that are not amenable for GC/MS analyses. The significance of the research could be far-reaching with respect to environmental concerns over black carbon. Teaching undergraduate and graduate research is a real strength of both PI's. The PI's demonstrated priority emphasis on hands-on experience, including research experiences for graduates and undergraduates in three different courses. The attitude that instrumentation should not be used as a 'black box' but rather as a hands-on learning opportunity pervades the text, and this is an important plus for this proposal. This aspect meets one of the principal NSF strategies for fostering the integration of research and education.