MRI-R2: Acquisition of equipment for investigating biodynamic interactions

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。在NSF MRI-R2项目的支持下，购买了Beckman Coulter MoFlo XDP细胞分选仪、BD Pathway 885高含量生物成像仪、Miltenyi Gentle mac解离器以及相关设备，以支持研究重点是了解导致细胞分化的分子相互作用的生物动力学。这一跨学科的努力整合了生物化学、免疫学、生理学、分子细胞生物学、神经生物学和植物生物学等学科的教师、博士后和学生研究人员。这套仪器计划的研究结果将允许识别、分离、培养特定的T细胞亚群，并实时成像它们与特定激活和抑制因子的相互作用。生长因子对在碳纳米管和磁性纳米管基质上生长的新分离背根神经节神经元亚群的差异影响将被表征。完整动物的肺细胞和免疫细胞对植物凝集素和荧光纳米晶体的吸收以及这种吸收对细胞分子动力学的后续影响将被表征。该设备还将用于支持对高中生、本科生和研究生的科学教育推广。亚利桑那州立大学有活跃的美国国家科学基金会资助的REU、URM、GK-12和路易斯·斯托克斯少数民族联盟项目，以及最近资助的麦克奈尔项目，重点是招募、留住和提高代表性不足的少数民族学生的教育经历。这些学生中有许多人积极参与pi实验室的研究项目。为了进一步扩大潜在的影响，除了将这些技术整合到我们的分子生物科学博士免疫学和显微镜技术课程中外，还将举办培训和应用研讨会。提高研究能力将加强大学的努力，以招收和留住少数民族学生、教师和合作者，否则他们可能会选择其他大学或其他学科进行研究。这些研究的结果将发表在同行评议的期刊上，并由学生和教师在区域和国家会议上介绍。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Support from the NSF MRI-R2 program has allowed the purchase of a Beckman Coulter MoFlo XDP cell sorter, a BD Pathway 885 High-Content Bioimager, and a Miltenyi Gentle Macs Dissociator, and related equipment to support research focused on understanding the biodynamics of molecular interactions leading to cellular differentiation. This interdisciplinary effort integrates faculty, post-doctoral and student researchers in the disciplines of biochemistry, immunology, physiology, molecular cell biology, neurobiology and plant biology. Results of studies planned for this suite of instruments will allow specific subsets of T cells to be identified, separated, cultured, and their interactions with specific activating and inhibiting factors imaged in real time. The differential effects of growth factors on freshly isolated subpopulations of dorsal root ganglion neurons grown on carbon and magnetic nanotube substrates will be characterized. The uptake of plant lectins and fluorescent nanocrystals by pulmonary and immune cells in intact animals and the subsequent effects of this absorption on cellular molecular dynamics will be characterized. The equipment will also be used to support science education outreach to high school, undergraduate and graduate students. ASU has active NSF-funded REU, URM, GK-12, and Louis Stokes Minority Alliance programs, as well as a recently funded McNair project, that focus on recruiting, retaining and enhancing the educational experience of students of under-represented minorities. Many of these students are actively participating in research projects in the PIs labs. To further broaden potential impacts, training and applications seminars will be held in addition to integrating these technologies into our Molecular Biosciences PhD techniques courses in immunology and microscopy. Enhanced research capabilities will strengthen the University's effort to recruit and retain minority students, faculty, and collaborators who might otherwise choose other universities or other disciplines to study. Results from the studies will be published in peer-reviewed journals and presented by students and faculty at regional and national meetings.