STTR Phase II: Engineering Clostridial Fermentation for Biobutanol Production

该STTR第二阶段项目将开发用于发酵的新型工程梭菌菌株，并从淀粉和木质纤维素生物质衍生的糖中经济地生产丁醇作为生物燃料。 传统的丙酮-丁醇-乙醇（ABE）发酵具有低丁醇产率（25%）、丁醇浓度（16 g/L）和反应器生产能力（0.5 g/L？h）由于丁醇具有强烈的抑制作用，并且由于生产微生物（主要是丙酮丁醇梭菌）中涉及复杂的代谢途径和基因调控，发酵过程难以改善。该项目更广泛的影响/商业潜力是从淀粉和木质纤维素生物质衍生的糖生产丁醇作为生物燃料。 生物丁醇作为替代运输燃料具有巨大的价值。 在提高丁醇产量和降低丁醇生产成本方面存在巨大的潜在商业和社会影响。所提议的研究和其他活动可能直接导致可销售的产品和工艺，并导致若干使能技术，包括更好地操纵C。Tyrobutylicum，使用FBB的菌株改进的进一步证明等。

This STTR Phase II project will develop novel engineered Clostridia strains for fermentation and economically produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. The conventional acetone-butanol-ethanol (ABE)fermentation has low butanol yield (25%), butanol concentration (16 g/L), and reactor productivity (0.5 g/L?h) due to a strong butanol inhibition, and the fermentation process is difficult to improve due to the complicated metabolic pathways and gene regulation involved in the production microorganisms, mainly Clostridium acetobutylicum.The broader impact/commercial potential of the project is to produce butanol as a biofuel from sugars derived from starchy and lignocellulosic biomass. Biobutanol has great value as an alternative transportation fuel. There is a huge potential commercial and societal impact in improving yields and reducing costs of butanol production. The research and other activity proposed could lead directly to a marketable product and process and leads to several enabling technologies, including better manipulation of C. tyrobutylicum, further demonstration of strain improvements using the FBB, and others.