Polyphasic studies on exiccata: phylogenetic evaluation of botanical type materials from historical Herbaria for cyanobacteria

首次将整合基因型和表型研究的多相分类法应用于蓝藻历史植物标本馆收藏。新的分子工具结合经典的分类学方法有望改善这种代谢均匀但形态复杂的微生物的分类基础。提出的项目将引入分子数据的经典描述模式物种沉积在植物标本馆。这样，植物学意义上的模式种也可以被微生物学家所接受。在这个项目中获得的数据将允许在统一两个方面迈出第一步：植物学和细菌学代码，至少在蓝藻的情况下。在此，我们提出基于分子方法和形态学数据的多相分类体系，为积累的历史蓝藻标本库分类知识提供一个新的框架。此外，将现有的毒性或固氮菌株与形态相同的类型材料进行分子比较，以测试基因型随时间的变化。该项目的新颖之处在于，一个模式标本将在其遗传和表型特性方面进行探索。这开启了验证大量生态学相关研究的可能性，并将现代多相多样性评估与传统的基于表型的鉴定和花卉列表联系起来。这将首次测试通过表型特征鉴定的微生物是否确实与传统假设的相同基因型相对应。还将检验以固定型标本为参考的生理学实践，还是以无菌活培养物为参考的细菌学实践更可靠。

For the first time polyphasic taxonomy integrating genotypic and phenotypic studies will be applied to historical herbarium collections of cyanobacteria. The new molecular tools combined with classical taxonomic procedures promise to improve the basis for classification of this metabolically uniform but morphologically complex microorganisms. The project proposed will introduce molecular data to classically described type species deposited in Herbaria. In that way type species in botanical sense could be also accepted by microbiologists. Data gained during this project would allow doing the first step in unification of two: botanical and bacteriological codes, at least in case of cyanobacteria. Here, we propose to introduce polyphasic taxonomy system based on molecular methods and morphological data to provide a new scaffold for the accumulated taxonomic knowledge on historical cyanobacterial herbaria. Furthermore molecular comparison of present e.g. toxic or nitrogen fixing strains with the morphologically identical, type-material will be performed in order to test the genotypic changes over time. The novelty of the project is that a type specimen will be explored in terms of its genetic as well as phenotypic properties. This opens the possibility to validate a large volume of ecologically relevant research and connect the modern polyphasic assessment of diversity with traditional phenotype-based identifications and floral listings. It will be tested for the first time whether microorganisms identified by phenotypic characters indeed correspond to the same genotype as traditionally assumed. It will also test whether the phycological practice of relying on fixed type specimens for reference or the bacteriological one using live axenic cultures as type reference are more reliable.