Acquisition, Assembly and Use of a High Speed Fluorescence Anisotropy and Lifetime Imaging System

通过化学研究仪器和设施：多用户（CRIF:MU）项目的奖励，密歇根州立大学的John McCracken教授和他的同事Gary Blanchard、Robert Ofoli、Greg Swain和David Weliky将获得一系列组件，以建立一个快速采集、皮秒荧光寿命和各向异性成像系统。该奖项将加强各级的研究培训和教育，特别是在以下研究领域：(a)脂质结构的荧光寿命和各向异性成像，(b)仿生纳米结构界面的光学和电化学表征，(c)石墨烯和碳薄膜研究的化学修饰电极，以及(d)病毒融合肽和蛋白质诱导的膜扰动成像。快速采集、皮秒级荧光寿命和各向异性成像系统对于研究生物膜等广泛的结构非均质界面和流体界面具有重要意义。这些实验提供了关于分子运动和能量传递的新信息，特别是这些特性如何随界面的物理条件和化学成分而变化。该仪器不仅将支持研究活动，还将为密歇根州立大学和附近机构（如萨吉诺谷州立大学）的研究生和本科生提供研究培训。该仪器还将支持与华沙大学、巴斯大学和陕西师范大学的国际互动，以及与当地高中生的活动。

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor John McCracken and colleagues Gary Blanchard, Robert Ofoli, Greg Swain and David Weliky from Michigan State University will acquire a series of components to build a rapid acquisition, picosecond fluorescence lifetime and anisotropy imaging system. The award will enhance research training and education at all levels, especially in areas of study such as (a) fluorescence lifetime and anisotropy imaging of lipid structures, (b) optical and electrochemical characterization of biomimetic nanostructured interfaces, (c) chemically modified electrodes for studies of graphene and thin carbon films, and (d) imaging of membrane perturbations induced by viral fusion peptides and proteins.A rapid acquisition, picosecond fluorescence lifetime and anisotropy imaging system is important to the study of a broad range of structurally heterogeneous and fluid interfaces such as those in biological membranes. These experiments provide novel information on molecular motion and energy transfer, and specifically how these properties vary with the physical condition and chemical composition of the interface. This instrumentation will support not only research activities but also research training to graduate and undergraduate students at Michigan State University and nearby institutions such as Saginaw Valley State University. The instrument will also support international interactions with the University of Warsaw, University of Bath and Shaanxi Normal University as well as activities with local high school students.