Dynamics of cell contacts during cell sorting at compartment boundaries in Drosophila

将增殖的组织划分为不混合的细胞群，称为隔室，对动物发育至关重要。隔间之间的尖锐和笔直的边界对于信号中心的定位以及因此对于生长和图案化是重要的。细胞分裂引起的细胞重排挑战，然而，直和尖锐的区室边界。我们最近的研究结果表明，差异肌球蛋白II依赖的机械张力对细胞连接是重要的，以保持直的车厢之间的接口。本项目的目的是了解这种差异性的机械张力如何引导细胞分裂后的细胞重排，以保持直的隔室边界。我们将结合联合收割机现场成像，荧光恢复后光漂白（FRAP），激光nanosurgery与定量图像分析，以确定和相关的肌球蛋白II马达蛋白的动力学和机械张力的细胞分裂沿着发展果蝇翅膀的前后室边界的动态细胞连接。将在使用肌球蛋白II突变体的实验中测试沿前后室边界的连接动力学沿着的功能意义。我们希望这个项目将提供新的机制的见解，指导细胞分选在动物发育过程中的细胞机制。

The partitioning of proliferating tissues into groups of non-intermingling cells, termed compartments, is crucial for animal development. Sharp and straight boundaries between compartments are important for the positioning of signalling centres and thus for growth and patterning. Cell rearrangements caused by cell division challenge, however, straight and sharp compartment boundaries. Our recent findings indicate that differential Myosin II-dependent mechanical tension on cell junctions is important for maintaining straight interfaces between compartments. The aim of this project is to understand how such a differential mechanical tension guides the rearrangement of cells after cell division to maintain straight compartment boundaries. We will combine live imaging, Fluorescence recovery after photobleaching (FRAP), and laser nanosurgery with quantitative image analysis to determine and to correlate the dynamics of cell junctions with the dynamics of the Myosin II motor protein and the dynamics of mechanical tension in cells dividing along the anteroposterior compartment boundary of the developing Drosophila wing. The functional significance of junctional dynamics along the anteroposterior compartment boundary will be tested in experiments using mutants for Myosin II. We expect that this project will provide novel mechanistic insights into the cellular mechanisms that guide cell sorting during animal development.