CAREER: A New Vivo Brain Circuit Analysis Method using Real-Time, High-Resolution Optogenetic fMRI

1056008lee该方案采用了一种全新的技术，称为光遗传功能磁共振成像（ofMRI）（由Lee博士首创），结合了先进的成像，计算和应用数学算法。光遗传学是一种遗传技术，可以根据神经回路元件的遗传特性和时间精度选择性地触发它们，而通带b-SSFP fMRI可以提供高质量的功能性脑激活图，MRI是一种将两者结合起来的技术，可以在选择性触发时看到大脑回路的精确因果反应。这使得大脑回路元素在系统级结果中的因果作用得以评估。然而，为了解析大脑回路反应的大量组合，实时交互监测是必要的，而大脑的小而密集的结构需要高分辨率的采集。PI旨在设计和实现新的并行计算和压缩感知（CS）重建算法来实现这些目标。该装置将被用来观察大脑回路对一系列频率刺激的反应。特别是，运动皮质（M1）、纹状体、丘脑和黑质（Stn）将受到刺激，以便精确地绘制已知与帕金森病运动症状相关的系统。然后仔细比较大脑活动的幅度、形状和分布来分析电路。

1056008LeeThis proposal utilizes a brand new technology called optogenetic fMRI (ofMRI)(pioneered by Dr. Lee) combined with advanced imaging, computation, and applied mathematical algorithms. Optogenetics, is a genetic technology that allows neural circuit elements to be triggered selectively based on their genetic identity with temporal precision, while passband b-SSFP fMRI, provides high quality functional brain activation maps to be obtained of MRI is a technology that combines the two to visualize precise causal response of the brain circuit upon selective triggering. This allows the brain circuit element's causal role in the system level outcome to be evaluated. However, in order to parse through the massive combination of the brain circuit's response, real-time interactive monitoring is necessary while the small, dense structures of the brain requires high resolution acquisition. The PI aims to design and implement novel parallel computation and compressed sensing (CS) reconstruction algorithms to achieve these goals. The implementation will then be utilized to look at brain circuit responses to a range of frequency stimulation. In particular, the motor cortex (M1), striatum, thalamus, and substantia nigra (Stn) will be stimulated in order to precisely map the system known to be correlated with the motor symptoms of Parkinson's disease. Brain activity amplitude, shape, and distribution will then be carefully compared to analyze the circuitry.