Gametogenesis-related small non-coding RNAs and Argonaute proteins in Arabidopsis (B05)

小rna和argonaute是基因调控的核心，但对开花植物卵细胞中这些核糖核蛋白（RNP）复合物的分析仍然具有挑战性，因为它深埋在卵巢内。B5项目旨在揭示拟南芥卵细胞的小RNA谱和RNP复合物，并研究卵细胞中的mirna是否以及如何影响细胞规格、受精或胚胎发生。此外，B5项目旨在阐明雌性配子体微环境中细胞间小RNA传递的分子机制。

Small RNAs and Argonautes are central to gene regulation but analysis of these ribonucleoprotein (RNP) complexes in the flowering plant egg cell remains challenging because it is deeply buried inside the ovary. Project B5 aims to uncover small RNA profiles and RNP complexes of Arabidopsis egg cells and to investigate whether and how miRNAs in the egg cell impact cell specification, fertilization or embryogenesis. Furthermore, project B5 aims to elucidate the molecular mechanism(s) of cell-to-cell small RNA transfer in the microenvironment of the female gametophyte for intercellular communication.