Collaborative Research: Analytical Method Transfer - Development of Case Studies

在这个合作项目中，圣约翰·费舍尔学院和肯纳索州立大学高级分析化学实验室课程的本科生正在创造两个新的实验，并通过分析方法转移(AMT)进行验证。一所学院的学生开发实验方法、标准操作程序和优值系数(即准确度、精密度、特异度、选择性、灵敏度、重复性、重复性、线性、范围、检测限、定量限、稳健性和坚固性)。实验被转移到合作学院，由学生进行方法等价性测试。实验包括药物剂型的溶出度和溶出度曲线的发展(释放率)。使用UV-Vis光谱或高效液相色谱方法，学生可以确定有效药物成分在溶解介质中的浓度。优秀度的统计比较决定了AMT的成功。转移的实验和学生生成的数据正在开发成使用POGIL练习的案例研究。与AMT相关的学生学习结果正在通过标准的前测和后测方法进行评估，方法是多项选择和自由回答问题。此外，其他四所大学的学生正在评估开发的POGIL活动，但没有使用仪器。收集的数据将被用来确定参加实验室POGIL活动(可以使用仪器)的学生的学习结果是否与参加课堂POGIL活动(不可以使用仪器)的学生的学习结果相同。正在进行学生访谈，以确定AMT是否有任何学习收益可能归因于POGIL活动。访谈结果提供了背景信息，使项目工作人员能够根据学生的经验量身定做POGIL活动。现场访问也被用来通过记录各种变量，如遵守项目目标和使用有效的POGIL，来评价POGIL活动的执行情况。该项目的发现正在通过出版物和演示文稿进行传播。AMT的教学将学生置于分析化学全球学习环境的前沿。学生1)使用目前大多数分析化学实验室没有的一种新仪器，即溶解测试仪，2)阐述AMT的课程概念，该课程在世界范围内进行，但目前不向本科生教授。这使学生和教职员工都参与到基于功绩数字的AMT验收标准的发现中。该项目旨在促进一个新的实验室实验在其他地点的改编，并促进机构间师生伙伴关系的合作努力。结果正在广泛传播，帮助建立STEM教育社区。学生的技术技能正在拓宽，使他们能够在全球市场上具有竞争力。

In this collaborative project undergraduate students enrolled in senior-level analytical chemistry laboratory courses at St. John Fisher College and Kennesaw State University are creating two novel experiments that are being validated through Analytical Method Transfer (AMT). Students at one college develop the experimental method, standard operating procedures, and figures of merit (i.e., accuracy, precision, specificity, selectivity, sensitivity, repeatability, reproducibility, linearity, range, detection limit, quantitation limit, robustness, and ruggedness). The experiment is transferred to the partnering college to undergo method equivalency testing by students. The experiments involve the dissolution of pharmaceutical dosage forms with the development of dissolution curves (release rates). Using UV-Vis spectroscopy or HPLC methods, the students determine the concentration of the active pharmaceutical ingredient in the dissolution media. A statistical comparison of the figures of merit determines the success of the AMT. The transferred experiments and student-generated data are being developed into case studies that use POGIL exercises. The student learning outcomes related to the AMT are being assessed through a standard pre-test and post-test method using multiple choice and free-response questions. In addition, students at four other colleges are evaluating the developed POGIL activities but are not using instrumentation. The collected data will be used to determine whether the learning outcomes for students participating in a laboratory POGIL activity (with access to instrumentation) are equivalent to those for students participating in a classroom POGIL activity (with no access to instrumentation). Student interviews are being conducted to determine whether any learning gains in AMT may be attributed to the POGIL activities. The interview results provide contextual information allowing project staff to tailor the POGIL activities to the students' experiences. Site visits also are being used to evaluate the fidelity of the implementation of the POGIL activities by documenting variables such as adherence to project goals and use of effective POGIL. The findings from the project are being disseminated through publications and presentations.Intellectual Merit. The teaching of AMT places students at the forefront of the global learning environment in analytical chemistry. Students 1) use a new instrument, a dissolution tester, not currently available in most analytical chemistry laboratories, and 2) address the curriculum concept of AMT, which is conducted world-wide but not currently taught to undergraduates. This engages both students and faculty in the discovery of acceptance criteria for an AMT based on figures of merit.Broader Impact. This project is designed to facilitate adaptation of a new laboratory experiment at other sites and promotes a collaborative effort of an inter-institutional faculty-student partnership. The results are being disseminated broadly, helping to build a STEM education community. The technical skills of students are being broadened, enabling them to become competitive in the global market place.