MRI: Acquisition of GC-MS to Support Basic and Applied Research and to Integrate Research and Education at Saint Cloud State University

有了这个来自主要研究仪器（MRI）计划的奖项，来自州立大学的圣克劳德的Latha Ramakrishnan教授及其同事Michael Jeannot、Mohammad Mahroof-Tahir、Lakshmaiah Sreerama和Mark Mechelke将获得一个气相色谱-质谱仪系统。该提案旨在加强各级的研究、培训和教育，特别是在以下领域：（a）了解涡虫中γ-氨基丁酸能神经传递的演变和功能;（B）确定废水处理中的5-羟色胺和去甲肾上腺素再摄取抑制剂;（c）验证顶空单滴微萃取动力学模型;（d）开发生物加工技术，从木质纤维素生物质生产乙醇;以及（e）设计和合成角硫硫胺素类似物以探索天然产物的作用机制。质谱仪蒸发并分离多组分混合物，然后将其传递到质谱仪中，在质谱仪中组分被电离。 质谱仪测定这些粒子（母体分子离子和碎片离子）的质荷比。这种高灵敏度的技术可以确定颗粒的质量，它们的化学成分，并使人们能够推断出复杂混合物中物质的结构。该奖项提供的仪器将为教师和学生提供在圣克劳德大学从事研究项目的机会。 它将促进许多学生在使用这一重要和广泛使用的分析技术的培训。

With this award from the Major Research Instrumentation (MRI) program, Professor Latha Ramakrishnan from St. Cloud State University and colleagues Michael Jeannot, Mohammad Mahroof-Tahir, Lakshmaiah Sreerama and Mark Mechelke will acquire a gas chromatograph-mass spectrometer system. The proposal is aimed at enhancing research training and education at all levels, especially in areas such as (a) understanding the evolution and function of GABAergic neurotransmission in planaria; (b) determination of serotonin and norepinephrine reuptake inhibitors in the wastewater treatment;(c) verification of a kinetic model for headspace single-drop microextraction; (d) development of bioprocess technologies to produce ethanol from lignocellulosic biomass; and (e) design and synthesis of goniothalamin analogues to explore the natural product mechanism of action.A gas chromatograph-mass spectrometer vaporizes and separates a multi component mixture which then passes into the mass spectrometer where the components are ionized. The mass spectrometer determines the mass/charge ratio of these particles (parent molecular ion and fragment ions). This highly sensitive technique allows determination of the masses of the particles, their chemical composition and enables one to deduce the structure of the substances in a complex mixture. The instrumentation provided by this award will provide faculty and students the opportunity to pursue research projects at St. Cloud University. It will advance the training of many students in the use of this important and widely used analytical technique.