MRI: Acquisition of a High Resolution GC-MS-MS with Fully Automated Sample Preparation to Advance Chemical Synthesis, Metabolomics, and Geochemical Research at IU Bloomington

MRI：购买高分辨率 GC-MS-MS 和全自动样品制备，以推进印第安纳大学伯明顿分校的化学合成、代谢组学和地球化学研究

• 批准号: 1726633
• 负责人: Jonathan Karty
• 金额: $ 43.75万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726633&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; GC; MS

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) programs. Professor Jonathan Karty from Indiana University and colleagues Simon Brassell, Silas Cook, James McKinlay and Jason Tennessen are acquiring a high resolution gas-chromatograph tandem mass spectrometer with tandem capabilities (HRes GC-MS-MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components go into a mass spectrometer where they are ionized and the ion masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a gas chromatograph provides additional structural identification power by separating gaseous mixtures of compounds before they reach the mass spectrometer. The tandem capabilities allow for additional separation in a second stage. This acquisition strengthens the research infrastructure at the University. The instrument broadens participation by involving diverse students in research and training using this modern analytical technique. It also provides training opportunities to many undergraduate, graduate and postdoctoral students from chemical, biological, geological and medicinal sciences as well as high school students through a variety of programs such as the Indiana University Summer Scholars Institute for students at minority serving institutions as well as the Louis Stokes Alliance for Minority Participation.This mass spectrometer enhances research and education at all levels. It enables research that elucidates of the role of climate on human evolution by measuring paleoclimate markers from Olduvai Gorge and explores the metabolic basis for cell proliferation and growth in Drosophila. The instrumentation aids in carrying out 13-carbon experiments to quantify metabolic pathway utilization in microbes as well as in distinguishing intermediates generated during organic syntheses of natural products. The mass spectrometer is also used in profiling and characterizing pheromones and sustainable carbon-carbon bond forming catalysts.

该奖项由主要研究仪器（MRI）和化学研究仪器（CRIF）计划支持。来自印第安纳州大学的Jonathan Karty教授及其同事Simon Mellell、塞拉斯库克、James McKinlay和Jason Tennessen正在购买一台具有串联功能的高分辨率气相色谱串联质谱仪（HRes GC-MS-MS）。一般来说，质谱（MS）是用于识别和表征复杂基质中少量化学物质的关键分析方法之一。在一个典型的实验中，成分进入质谱仪，在那里它们被电离，并测量离子质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。带有气相色谱仪的仪器通过在化合物的气体混合物到达质谱仪之前分离它们来提供额外的结构鉴定能力。串联能力允许在第二级中进行额外的分离。此次收购加强了大学的研究基础设施。该仪器通过让不同的学生参与使用这种现代分析技术的研究和培训来扩大参与。它还通过各种计划为化学、生物、地质和医学科学的许多本科生、研究生和博士后学生以及高中生提供培训机会，例如为少数民族服务机构的学生提供的印第安纳州大学夏季学者研究所以及路易斯斯托克斯少数民族参与联盟。它通过测量奥杜威峡谷的古气候标志物来阐明气候对人类进化的作用，并探索果蝇细胞增殖和生长的代谢基础。该仪器有助于进行13碳实验，以量化微生物中的代谢途径利用，以及区分天然产物有机合成过程中产生的中间体。质谱仪还用于分析和表征信息素和可持续的碳-碳键形成催化剂。

项目成果

A Drosophila model of combined D-2- and L-2-hydroxyglutaric aciduria reveals a mechanism linking mitochondrial citrate export with oncometabolite accumulation.

• DOI: 10.1242/dmm.035337
• 发表时间: 2018-09-21
• 期刊: Disease models & mechanisms
• 影响因子: 4.3
• 作者: Li H;Hurlburt AJ;Tennessen JM
• 通讯作者: Tennessen JM

Methods for studying the metabolic basis of Drosophila development.

• DOI: 10.1002/wdev.280
• 发表时间: 2017-09
• 期刊: Wiley interdisciplinary reviews. Developmental biology
• 作者: Li H;Tennessen JM
• 通讯作者: Tennessen JM

Iron-Catalyzed Hydroamination and Hydroetherification of Unactivated Alkenes

• DOI: 10.1021/acs.orglett.9b00427
• 发表时间: 2019-03-01
• 期刊: ORGANIC LETTERS
• 影响因子: 5.2
• 作者: Marcyk, Paul T.;Cook, Silas P.
• 通讯作者: Cook, Silas P.

The oncometabolite L-2-hydroxyglutarate is a common product of dipteran larval development.

• DOI: 10.1016/j.ibmb.2020.103493
• 发表时间: 2020-12
• 期刊: Insect biochemistry and molecular biology
• 影响因子: 3.8
• 作者: Mahmoudzadeh NH;Fitt AJ;Schwab DB;Martenis WE;Nease LM;Owings CG;Brinkley GJ;Li H;Karty JA;Sudarshan S;Hardy RW;Moczek AP;Picard CJ;Tennessen JM
• 通讯作者: Tennessen JM

Synthesis of Tetrahydroisoquinolines Through an Iron-Catalyzed Cascade: Tandem Alcohol Substitution and Hydroamination

• DOI: 10.1021/acs.orglett.9b02353
• 发表时间: 2019-09-06
• 期刊: ORGANIC LETTERS
• 影响因子: 5.2
• 作者: Marcyk, Paul T.;Cook, Silas P.
• 通讯作者: Cook, Silas P.