Genetically controlled self-assembly of inorganic-bioorganic hybrid structures: From sponge genes to layered functional materials

拟议的项目旨在基于无机多组分氧化物和重组硅酸盐蛋白的合成材料及其用于制造分层结构可调混合生物材料的评估。我们建议利用重组silicatein的生物催化合成的二元和多元（例如SnO 2，In 2 O3，BaSnO 3，PbTiO 3，LaFeO 3）氧化物和制造的多层结构从交替的蛋白质/氧化物层。或者，布拉格堆栈将被用作这些多层混合的合成类似物。将通过噬菌体展示筛选来选择对各种钙钛矿（例如BaSnO 3、BaTiO 3、BiFeO 3、PbTiO 3）具有特异性亲和力的肽。此外，应制备含有硅酸盐蛋白、钙钛矿-仿射肽和基于肽序列的自组装结构域的聚合物，所述肽序列导致形成互连的自组装β-折叠原纤维或聚-（N-异丙基-丙烯酰胺）（PNIPAM）聚合物，所述聚合物形成在较低临界溶液温度下经历体积相变的刺激响应性聚合物。这些新的杂化聚合物将研究它们的自组装性能，它们的溶液行为，如较低的临界溶液温度，它们形成凝胶沉淀的能力和其他。同时，将评估silicatein蛋白质聚合物用于多金属钙钛矿和类钙钛矿材料的成核和生长的潜力，这些材料具有广泛的有用的电子、磁性和光学性质。

The proposed project aims at the synthesis materials based on inorganic multicomponent oxides and recombinant silicatein and their evaluation for the fabrication of hierarchically structured tunable hybrid-biomaterials. We propose to utilize recombinant silicatein for the biocatalytic synthesis of binary and multinary (e.g. SnO2, In2O3, BaSnO3, PbTiO3, LaFeO3) oxides and the fabrication of multilayered structures from alternating proteins/oxide layers. Alternatively, Bragg stacks shall be used as synthetic analogues of these multilayer hybrids. Peptides with a specific affinity to various perovskites (e.g. BaSnO3, BaTiO3, BiFeO3, PbTiO3) will be selected by phage display screening. In addition, polymers containing silicatein, perovskite-affine peptides and self-assembling domains based on peptide sequences leading to the formation of interconnected, self-assembled β-sheet fibrils or poly-(-N-isopropyl-acrylamide) (PNIPAM) polymers that form stimulus-responsive polymers undergoing a volume phase transition across a lower critical solution temperature shall be prepared. These novel hybrid polymers will be investigated for their self-assembly properties, their solution behavior such as lower critical solution temperatures, their ability to form gel precipitates and others. In parallel, the potential of the silicatein protein polymers for the nucleation and growth of multimetallic perovskite and perovskite-like materials will be evaluated that possess a wide range of useful electronic, magnetic, and optical properties.