Bio-inorganic hybrid membranes with nanoporosity control by genetically engineered viral seal rings

提出了一种制备纳米级孔阵列生物/无机杂化膜的新方法。这个过程可以控制毛孔之间的距离以及它们的直径。为此，将开发一种病毒模板辅助的固态膜方法。具体来说，将使用烟草花叶病毒（TMV）组装中间体形成精确尺寸的蛋白质圆盘，其内孔为4nm，外径为18nm。这些中间体可能在基因上既适合于内孔表面，也适合于外缘表面。因此，将功能化无机模板（MT）与TMV“孔适配器嵌体”相结合，可能会产生既具有稳定的无机骨架又具有接近于零尺寸偏差的纳米孔的膜。预期的实验策略是通过仿生“胶水”将生物相和无机相连接起来：肽介导的与固体二氧化硅的相互作用和随后的蛋白质诱导的二氧化硅沉积应该固定mt中的TMV盘并密封间隙。主要目标是生产具有特定定位，功能化锥形孔（Ø 15-30 nm）和基因定制的三功能TMV蛋白盘的层状膜，通过中间化学偶联将其定位并预先固定到孔中，并通过在两种材料相之间形成的崎岖的生物矿物脊完成组装。分析了复合膜材料的质量和透气性。

A novel preparation route is proposed to obtain bio/inorganic hybrid membranes with arrays of nanoscaled pores. The procedure allows controlling the distance between pores as well as their diameter. For that purpose, a virus template-assisted solid state membrane approach will be de-veloped. Specifically, Tobacco mosaic virus (TMV) assembly intermediates forming protein disks of precise dimensions with an inner 4 nm pore and an outer diameter of 18 nm will be used. These inter-mediates may be genetically tailored on both, the inner hole and the outer rim surfaces. Hence, combining functionalized inorganic templates (MT) with TMV "pore adapter inlays" might yield mem-branes benefiting from both a stable inorganic backbone and nanopores with near-to-zero size devi-ations. The intended experimental strategy interconnects biogenic and inorganic phases by a bionic „glue‟: peptide-mediated interaction with solid silica and subsequent protein-induced SiO2 deposition should immobilize the TMV disks in MTs and seal gaps. Primary goals are to produce layered membranes with specifically addressable, functionalized conical pores (Ø 15-30 nm) and genetically tailored trifunctional TMV protein disks, to position and pre-fix them into pores by help of intermediate chemical coupling, and to finish the assemblies by rugged biogenic mineral ridges formed between the two material phases. Quality and permeability of the hybrid membrane materials will be analyzed.