New Frameworks of SSNMR for Biomolecules and Nano-assembled Systems for Sensitivity, Reaction, and Structure

核磁共振波谱是测定分子结构不可缺少的工具。在化学学部化学测量与成像项目的支持下，伊利诺伊大学芝加哥分校的Yoshitaka Ishii教授及其研究小组将建立固体中生物分子和材料的下一代核磁共振波谱新框架，通常称为固态核磁共振（SSNMR）。通过将1H检测、新型同位素标记和顺磁掺杂等先进方法集成到非传统的SSNMR方法中，利用超高磁场和非常快的魔角旋转，将数据收集速度提高到传统方法的50-6000倍。研究的第二个组成部分是对固相肽合成过程中的肽和蛋白质进行SSNMR分析，揭示反应过程中蛋白质的结构变化。第三部分是研究石墨烯/石墨基纳米材料，特别是氧化石墨烯和氧化石墨的化学反应以及水与多孔石墨烯基体系的相互作用。石井的研究将推动核磁共振技术应用于更广泛的不溶性蛋白质和非晶体材料，这些材料无法通过其他工具（如x射线晶体学）进行分析。石井小组的研究进展可能会对基于核磁共振技术的许多领域产生影响，包括纳米材料分析和肽科学。石井教授将把他的研究整合到他的教育活动中，通过建立一个新的研究生课程，固态核磁共振与动手实验。石井教授亦会在本科生实验室演示简单的核磁共振成像（MRI）实验与核磁共振场梯度的分子扩散实验，以探讨核磁共振与核磁共振技术用于医学诊断的联系。最后，石井教授将通过伊利诺伊大学芝加哥分校的本科生暑期研究机会项目（SROP）招募本科生研究人员，该项目强调研究型本科教育的多样性。

Nuclear magnetic resonance (NMR) spectroscopy is an indispensable tool for determining the structure of molecules. In this project, supported by the Chemical Measurement and Imaging Program of the Division of Chemistry, Prof. Yoshitaka Ishii of the University of Illinois at Chicago and his research group will establish a new framework of next-generation NMR spectroscopy for biomoleculces and materials in solids, which is generally called solid-state NMR (SSNMR). The proposed work would aim to increase the speeds of the data collection 50-6000 fold over a traditional method by integrating advanced methods such as 1H detection, novel isotope labeling, and paramagnetic doping into a non-traditional SSNMR approach using an ultra high magnetic field and very fast magic angle spinning. The second component of the research involves SSNMR analysis of peptides and proteins during solid-phase peptide synthesis, which reveals the structural changes of the protein during the reactions. The third component is to examine graphene/graphite based nano materials, in particular, chemical reactions of graphene oxide and graphite oxide and the interactions of H2O with porous grapheme-based systems. Ishii's research will advance NMR technology applications to a broader range of insoluble proteins and non-crystalline materials, which cannot be analyzed by other tools such as x-ray crystallography. Advances in the Ishii group research will likely have impacts in the many venues of NMR based technology, including nanomaterials analysis and peptide science. Professor Ishii will integrate his research into his educational activities by establishing a new graduate course on solid-state NMR with hands-on experiments. Professor Ishii will also incorporate a demo of a simple magnetic resonance imaging (MRI) experiment into molecular diffusion experiments with NMR field gradient in undergraduate labs in order to discuss the connection of NMR with the MRI technology used for medical diagnostics. Finally, Professor Ishii will recruit undergraduate researchers through Summer Research Opportunities for Undergraduates Program (SROP) at the University of Illinois at Chicago, which emphasizes the diversity in research-based undergraduate education.