STTR Phase I: Real time detection for salmonella

这个小型企业技术转移计划(STTR)第一阶段项目将创建一种新的沙门氏菌传感器，将两种已有的生物检测工具结合在一起：流体动力层析和磁性纳米颗粒(MP)结合。与目前的技术相比，所提出的传感器将大大降低成本，并在同等灵敏度下提供更快的检测时间。该项目将通过利用MP磁取向、流体行为和体积特性之间的联系来开发这种传感器。功能涂层的MPS将在微流控通道中与沙门氏菌适配子生物标记物结合。将使用超灵敏的巨磁电阻传感器测量溶液的磁性分布。由于MPS的修饰，在生物标记物存在的情况下，MP磁谱将发生变化。水动力体积。第一阶段计划的目标将是展示磁力分布的这种差异。该项目的成功完成将导致第二阶段工作中用于食品安全应用的传感器原型。该项目的更广泛影响/商业潜力可能包括食品安全和医疗应用。在相当大的食品加工业中，传感器是一种高效的污染监测器，通过防止受污染产品的分发，极大地改善了食品安全。虽然最初的传感器将针对沙门氏菌而设计，但该传感器也可扩展到其他生物标志物，并可被设想为医疗行业的关键护理点传感器。国家科学基金会？S的使命也将通过让研究生和博士后研究人员参与该项目来实现，让他们接触到行业，同时发展新知识和磨练他们的研究技能。

This Small Business Technology Transfer Program (STTR) Phase I project will create a new salmonella sensor combining two established tools in biodetection: hydrodynamic chromatography and magnetic nanoparticle (MP) conjugation. The proposed sensor will be significantly less expensive and provide faster detection time with equivalent sensitivity compared to current techniques. The project will develop this sensor by exploiting the connection between the MP magnetic orientation, fluid behavior and volume characteristics. Functionally coated MPs will be combined with a salmonella aptamer biomarker in a microfluidic channel. A magnetic profile of the solution will be measured using ultrasensitive giant magnetoresistance sensors. The MP magnetic profile will change in the presence of the biomarker due to modification of the MPs? hydrodynamic volume. The goal of the Phase I program will be to demonstrate this difference in magnetic profiles. Successful completion of the program will result in a prototype sensor for food safety applications in the Phase II effort.The broader impact/commercial potential of this project could include food safety and medical applications. In the sizeable food processing industry the sensor is a highly efficient contamination monitor leading to dramatic improvements in food safety by preventing distribution of contaminated products. While the initial sensor will be designed for salmonella, the sensor is also extensible to other biomarkers and could be envisioned as a critical point-of-care sensor in the medical industry. The National Science Foundation?s mission will also be carried out by having graduate students and postdoctoral researchers participate in the program, exposing them to industry while developing new knowledge and honing their research skills.