Communication between blood vessels and hepatic cells during liver regeneration (A01)

该项目的工作假设是，部分肝切除术（PHx）后，增强的血流拉伸肝内皮细胞，从而释放内皮细胞衍生的生长因子，以启动肝脏生长。相反，作用于内皮的增强的剪切应力导致生长抑制因子的释放，其停止肝脏生长并可导致肝损伤。在这里，机械调控的基因和分泌的蛋白质将在肝内皮细胞，机械分析，并在体外和体内功能的特点。

The working hypothesis of this project is that after partial hepatectomy (PHx) an enhanced blood flow stretches the hepatic endothelial cells and thereby releases endothelial cell-derived growth factors to initiate liver growth. Conversely, an enhanced shear stress acting on the endothelium results in release of growth-inhibiting factors that stop liver growth and can lead to liver damage. Here, mechanically regulated genes and secreted proteins will be identified in hepatic endothelial cells, mechanistically analysed, and functionally characterized in vitro and in vivo.