Synthesis and analytics of HHDPs

血红素降解产物负责各种生理活动。然而，我们对这些代谢物的转化机制和活性知之甚少。我们的知识仅限于血红素降解为胆红素和胆绿素的早期步骤以及具有特征的胆红素氧化最终产物(BOX)。本项目旨在阐明血红素降解的机理，为活性测试提供和表征中间体和最终产物，并跟踪体外和体内的降解过程。因此，我们将结合分析和合成化学工具来探索迄今已确定的代谢物以外的血红素降解途径。活性中间体将被捕获，制备型高效液相色谱法将作为纯化这些中间体的工具，以进一步阐明结构。为了验证结构和反应性，并为活性测试提供材料，将进行选定代谢物的合成。一个特别的焦点将是这些化合物在生物组织中的命运。

Heme degradation products are responsible for various physiological activities. However, we know little about the transformation mechanisms and activities of these metabolites. Our knowledge is limited to early steps in heme degradation towards bilirubin and biliverdin as well as to the characterized bilirubin oxidation end products (BOXes). This project aims to clarify the mechanisms of heme degradation, to provide and characterize intermediates and end products for activity tests, and to follow the degradation processes in vitro and in vivo. We therefore will combine analytical and synthetic chemical tools to explore the pathways of heme degradation beyond the hitherto identified metabolites. Reactive intermediates will be trapped and preparative HPLC will serve as a tool for the purification of these intermediates for further structure elucidation. In order to verify structure and reactivity and to provide material for activity testing, the synthesis of selected metabolites will be performed. A special focus will be the fate of the compounds in biological tissues.