Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells

Pi：Ioana Pavel Sizemore/Adam de la Zerda Proposal编号：1438340/1434465机构：莱特州立大学/斯坦福大学标题：合作建议：开发一系列方法来研究银和金纳米材料在人类细胞中的贩运机制和转化，在消费品和工业中广泛使用银纳米粒子(AgNP)，以及在医疗保健中使用多种NPs作为诊断和治疗工具，需要了解它们的潜在毒性。然而，根据国家纳米技术倡议的观察，只有有限的技术可以扩大我们对纳米材料影响细胞行为的机制的理解。该项目的主要科学目标是通过开发一系列工具来填补这些知识空白，这些工具用于研究具有环境代表性的银纳米粒子和与生物医学相关的金纳米棒在人体细胞中的贩运机制和转化。将考虑的关键参数将包括纳米材料的形状、表面电荷、剂量和曝光时间，以测试所建议方法的有效性和实用性。将通过标准操作程序(SOP)获取和共享要开发的方法。此外，从拟议研究中获得的知识将用于建立新的课程单元，并将传播到更广泛的社区，例如，通过与当地博物馆的互动，以及为100多名K-6至K-9学生开发两个小时的动手实验室单元。将使用一系列跨学科方法(ICP-OES/TFF、拉曼-SERS/MATLAB代码和电子显微镜/生物分析)来研究具有环境代表性的银纳米颗粒(AgNPs)和与生物医学相关的金纳米颗粒(AuNRs)在人体细胞中的传输机制和转化。将考虑的关键参数将测试提议的方法的效率和实用性，包括纳米材料的形状、表面电荷、剂量和曝光时间。为了实现他们的目标，私人投资机构提出了两个具体目标。在AIM1中，PI将根据美国环保局的标准，在细胞暴露之前以及在细胞贩运期间的不同基准时间表征NPs的物理化学性质。在目标2中，研究人员将使用他们的工具套件，研究AgNPs和AuNRs在人类细胞中的传输机制，作为纳米材料形状、表面电荷、剂量和暴露时间的函数。

PI: Ioana Pavel Sizemore/Adam de la ZerdaProposal Number: 1438340/1434465Institution: Wright State University/Stanford University Title: Collaborative proposal: Developing a battery of methods for the study of the trafficking mechanisms and transformations of silver and gold nanomaterials in human cells The extensive use of silver nanoparticles (AgNPs) in consumer products and industry and the use of many kinds of NPs in healthcare as diagnostic and therapeutic tools require an understanding of their potential toxicities. However, as observed by the National Nanotechnology Initiative, there are only a limited number of techniques that could expand our understanding of the mechanisms by which nanomaterials influence cell behavior. The main scientific goal of this project is to fill these knowledge gaps by developing a battery of tools for the study of the trafficking mechanisms and transformations of environmentally representative AgNPs and biomedically relevant gold nanorods (AuNRs) in human cells. Key parameters, which will be considered in order to test the efficiency and practicality of the proposed methods, will include nanomaterial shape, surface charge, dose, and exposure time. The methods that are to be developed will be captured and shared through Standard Operating Procedures (SOPs). In addition, the knowledge gained from the proposed research will be used in setting up new course modules and will be disseminated into the broader community, e.g., through interactions with a local museum and development of two hour hands-on laboratory modules for over 100 K-6 through K-9 students. A battery of interdisciplinary methods (ICP-OES/TFF, Raman-SERS/MatLab codes, and electron microscopy /bioassays) will be used for the study of the trafficking mechanisms and transformations of environmentally representative silver nanoparticles (AgNPs) and biomedically relevant gold nanoparticles (AuNRs) in human cells. Key parameters that will be considered to test the efficiency and practicality of the proposed methods will include nanomaterial shape, surface charge, dose, and exposure time. To accomplish their goals, The PIs propose two specific aims. In Aim1, the PIs will characterize the physico-chemical properties of NPs, according to the U.S. EPA standards, before cellular exposure and then at various benchmark times during cellular trafficking. In Aim 2, using their suite of tools, the investigators will study the trafficking mechanisms of AgNPs and AuNRs in human cells as a function of nanomaterial shape, surface charge, dose, and exposure time.