The role of Drosophila melanogaster condensin I subunits during male meiosis

异五粒凝集素复合体参与了有丝分裂和减数分裂染色体凝聚结构的建立和维持。虽然在脊椎动物中，两种凝聚素复合体，凝聚素I和凝聚素II，在有丝分裂和减数分裂中的作用已经得到很好的证实，但在果蝇中，发散的凝聚素II复合体不是有丝分裂增殖所必需的。然而，凝聚素II特异基因Cap-H2和Cap-D3的突变会导致卵子发生、雄性减数分裂和精子发生过程中的缺陷。凝聚素I特异性成分在雄性减数分裂和精子发生过程中的可能作用尚未被研究。然而，编码凝集素I特异性亚单位的基因在睾丸中表达。因此，有人建议通过RNAi或靶向蛋白水解性切割来特异性地去除睾丸中的凝集素I功能，并确定男性减数分裂和/或精子染色质成熟过程中可能的表型。

The heteropentameric condensin complex has been shown to be involved in establishing and main-taining the condensed structure of mitotic and meiotic chromosomes. While in vertebrates the role of two condensin complexes, condensin I and condensin II, has been well established during both mito-sis and meiosis, the diverged condensin II complex in Drosophila is not required for mitotic prolifera-tion. However, mutations in the condensin II-specific genes Cap-H2 and Cap-D3 result in defects dur-ing oogenesis, male meiosis and spermatogenesis. Possible roles for the condensin I-specific components in male meiosis and during spermatogenesis have not been investigated. However, the genes encoding condensin I-specific subunits are expressed in testes. Thus, experiments are proposed to specifically remove condensin I function in testes via RNAi or targeted proteolytic cleavage and to determine possible phenotypes during male meiosis and/or sperm chromatin maturation.