Impact of crosstalk phenomena on the defense cis-regulatory landscape in Arabidopsis visualized with DNase-seq footprinting.

为了理解转录因子（TFs）及其同源的顺式元件之间的大量可能的组合相互作用如何协调植物激素信号通路，需要对顺式调控景观有一个全面的看法。该提案要求资助利用dna -seq足迹在模式植物拟南芥中动态绘制TF在体内的足迹，重点是防御反应中的激素串扰。植物在病原体感染后重新合成称为植物激素的小信号分子，以建立适当的免疫状态。根据攻击者的生活方式，不同的激素被用来启动适当的防御反应。水杨酸（SA）的产生是为了有效地对抗生物营养病原体，茉莉酸（JA）和乙烯（ET）的产生是对抗坏死性病原体所必需的。当多种不同生活方式的病原菌同时感染或病原菌引起的防御激素稳态紊乱时，植物需要激素通路之间有效的串扰机制来微调防御努力，避免适应性损失。对国防相声机制的机械见解仍然有限。因此，为了阐明JA、ET和SA信号之间的串扰现象是如何在TF水平上执行的，本项目将在不同串扰条件下对全基因组水平的顺式调控结构进行首次动态调查。

To understand how the plethora of possible combinatorial interplays between transcription factors (TFs) and their cognate cis-elements coordinates plant hormone signaling pathways, a comprehensive view of the cis-regulatory landscape is required. This proposal requests funding for the dynamic mapping of TF in vivo footprints in the model plant Arabidopsis thaliana utilizing DNase-seq footprinting with an emphasis on hormone crosstalk in defense responses. Plants de novo synthesize small signaling molecules called plant hormones upon pathogen infection in order to establish an adequate status of immunity. Depending on the lifestyle of the attacker, different hormones are employed to initiate the appropriate defense responses. Whereas salicylic acid (SA) is produced to effectively counteract biotrophic pathogens, the production of jasmonic acid (JA) and ethylene (ET) is essential to combat against necrotrophic pathogens. In case of concomitant infection of multiple pathogens with different lifestyles or pathogen-induced disturbances of the defense hormone homeostasis, plants require efficient crosstalk mechanisms between the hormone pathways to fine-tune their defense efforts and to avoid fitness losses. Mechanistic insights into the defense crosstalk machinery are still limited. To elucidate therefore how crosstalk phenomena between JA, ET and SA signaling are executed on the TF level, this project will generate the first dynamic surveys of the cis-regulatory architecture at the whole genome-level under different crosstalk conditions.