Co-ordinate regulation of sugar, ethylene and hypoxia responses at the levels of transcription and metabolism by the SnRK1 kinase in Arabidopsis

AMPKs在真核生物的碳代谢、能量动态平衡、糖和胁迫信号以及转录等方面发挥着重要的调节作用。高等植物AMPK同源蛋白被称为Snf1相关蛋白(Snf1-Related Kinase，SnRK1)，被认为具有类似的功能，但到目前为止，人们对其在代谢、胁迫、激素和发育途径调控中的确切作用知之甚少。拟议的合作项目旨在将生理和代谢组学研究的专业知识与遗传、生化和转录组学方法结合起来，以便准确地剖析SnRK1在细胞调控中的主要功能。该项目的最终目标是确定SnRK1控制淀粉代谢和对低氧胁迫反应的机制，并将其与SnRK1在控制全基因组转录和乙烯/糖和ABA激素信号通路中的新角色联系起来。

AMP-activated protein kinases (AMPKs) play a central role in the regulation of carbon metabolism, energy homeostasis, sugar and stress signalling, and transcription in eukaryotes. Higher plant AMPK orthologs, named Snf1-related kinases (SnRK1), are proposed to perform analogous functions but so far little is know about their exact roles in modulation of metabolic, stress, hormonal and developmental pathways. The proposed collaborative project aims at joining expertise in physiological and metabolomics studies with genetic, biochemical and transcriptomics approaches in order to dissect precisely major functions of SnRK1 in cellular regulation. Ultimate goal of the project is to identify the mechanisms by which SnRK1 controls starch metabolism and stress responses to hypoxia, and to link these to emerging roles of SnRK1 in the control of genome-wide transcription and ethylene/sugar and ABA hormone signalling pathways.