Multifunctional Nanophases for Tunable Separations and Integrated Processing

在化学系化学测量和成像（CMI）项目的支持下，以及化学、生物工程、环境和运输系统（CBET）部门的共同资助下，西弗吉尼亚大学的Lisa Holland教授正在开发用于蛋白质分离的新材料，以阐明生物分子的细微变化，其应用范围从筛选酶抑制到鉴定翻译后蛋白质修饰。为了应对这一挑战，自调节磷脂纳米相被用于分类、筛选或识别生物分子的特定性质。更广泛的影响是通过支持化学研究生的专业发展来解决的，这些研究生参与创新和跨学科的研究经验，获得博士学位，最终进入劳动力市场，具有跨科学学科的工作能力。该奖项还支持一项推广活动，以个性化化学体验和提高公众科学素养。该项目专注于热可逆的磷脂纳米相，它作为化学筛分剂和粘度添加剂具有很高的用途，可以利用基于形状的摩擦阻力差异。研究了调节蛋白质选择性的各种添加剂。该项目建立了用于蛋白质筛选和多功能分离的纳米相的条件和基本性质。霍兰德教授在当地学校开展了一项拓展计划，使教师能够将低成本的微型实验纳入化学入门教育。通过记录廉价的设备设计、吸引教育供应商、开发课程和报告教育评估，其他教师可以使用这些产品。

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, and co-funding from the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET), Professor Lisa Holland of West Virginia University is developing new materials for protein separations to elucidate the subtle changes in biomolecules for applications ranging from screening enzyme inhibition to identifying post-translational protein modifications. To meet this challenge, self-tuning phospholipid nanophases are adapted to sort, screen, or identify specific properties of biomolecules. The broader impacts are addressed through the support of the professional development of chemistry graduate students who participate in innovative and interdisciplinary research experiences earning PhD degrees and ultimately entering the workforce with the ability to work across scientific disciplines. The award also supports an outreach activity to personalize chemistry experiences and to increase public science literacy. This project focusses on thermally reversible phospholipid nanophases that have high utility as a chemical sieving agent and as a viscosity additive to exploit shape-based differences in frictional drag. Various additives to tune protein selectivity are examined. The project establishes the conditions and fundamental properties of the nanophases for protein sieving and multifunctional separations. An outreach program developed by Professor Holland at area schools enables teachers to incorporate low-cost microscale experiments into introductory chemistry education. The products are made accessible to other teachers by documenting inexpensive device design, engaging educational suppliers, developing the curriculum, and reporting the educational assessment.