UNS:ADVANCING CELL-PRESERVING SEPARATION VIA DETACHABLE CELL ANCHORING & SPIRAL MICRO-MIXING

[12598]细胞分离对于推进个体化癌症药物尤其必要，因为肿瘤包含不同生物标志物的细胞的多样化组合，这使得它们难以治疗。如果不同的肿瘤细胞类型可以被分离和分析，那么根据患者肿瘤的独特特征定制药物的前景可能会得到分析。提出的研究目标是在微尺度上设计一种快速、温和的细胞分离系统。将细胞捕获到表面上，并通过靶向锚内的次级片段来释放细胞的能力将使细胞分离更加温和。因此，提出的研究旨在将螺旋微混合与细胞锚定和二级片段靶向细胞释放相结合，从而使细胞与血液分离同时保持受体。具体研究目标如下：(1)确定可分离胞体锚固的最佳条件；(2)确定可拆卸槽型锚定的螺旋微型混合器设计参数；(3)开发微流控平台，分离血液样本中的循环内皮细胞、免疫细胞和肿瘤细胞，同时保留分离细胞的表面受体。拟议的工作可能会开发创新技术，推动科学发展，同时支持高影响力的教育使命。

1512598ImoukhuedeUof Ill Urbana-ChampaignCell separation is especially necessary to advance personalized cancer medicine, because tumors contain a diverse mix of cells with different biomarkers that makes them difficult to treat. If different tumor cell types can be separated and analyzed the promise of tailoring drugs to the unique characteristics of a patient's tumor may be analyzed. The proposed research aims to engineer a fast and gentle cell separation system on a microscale. The ability to capture cells onto a surface and release by targeting a secondary moiety within the anchor would enable gentler, cell separation. As such, the proposed research aims to combine spiral micro-mixing with cell anchoring and secondary moiety targeted cell release to enable a cell separation from blood that is also receptor-preserving. The specific research goals are as follows: (1) identify the optimum conditions for detachable cell-anchoring; (2) determine the spiral micro-mixer design parameters enabling detachable cell-anchoring; (3) develop the microfluidic platform that will separate circulating endothelial cells, immune cells, and tumor cells from blood samples while preserving surface receptors on isolated cells. The proposed work may develop innovative technology that advances science while championing a high-impact, educational mission.