EAGER: An Integrated Multi-Sensing Platform for Real Time Hypoxia Studies

1550976 Agrawal， Nitin溶解氧是细胞必需的营养物质。它是少量可溶的，因此其浓度在实体肿瘤和相应的生理反应组织中变化。本提案试图创新一个传感平台，将测量细胞反应在不同的氧浓度在微流控排列。一个例子应用是剂量依赖性的细胞反应可以在不同水平的氧张力下有效地获得。溶解氧（DO）是哺乳动物细胞能量代谢所必需的营养物质。当细胞长成肿瘤时，溶解氧梯度在空间上发生变化。类似的情况也出现在没有很好血管化的组织和肌肉中。由于溶解氧对细胞生理有显著影响，因此需要一种测量和实验平台来详细表征不同溶解氧水平和溶解氧梯度下的细胞反应。为了解决这个问题，提出了在单个半自动平台上同时感知缺氧扰动和测量细胞反应的方法。集成的多传感平台能够施加线性氧梯度，而不需要连续的氧气供应。通过集成式薄膜PdO-EPK氧传感器实时监测溶解氧张力，连续监测细胞周期进程，分析多种药物对细胞增殖和存活的剂量效应。模型实验采用MDA-MB-231（乳腺）和U87MG（胶质母细胞瘤）两种细胞类型。从科学上讲，如果成功的话，提出的方法将创建一个进行氧张力研究的测量平台。该研究项目是多学科的，整合了生物工程、细胞生物学、微制造、计算设计和建模，将为全日制博士生提供一个有趣的学习机会。计划通过乔治梅森大学将STEM领域的多学科培训纳入其中。s (GMU) ？有抱负的科学家暑期实习计划？旨在促进弗吉尼亚州北部高中和本科生的研究和教育。通过对传感器平台的拟议研究，pi将通过？路易斯·斯托克斯少数民族参与联盟？GMU的项目。

1550976 Agrawal, Nitin Dissolved oxygen is an essential nutrient for cells. It is sparingly soluble and thus its concentration varies in solid tumors and tissues with consequential physiological response. This proposal attempts to innovate a sensing platform that will measure cellular response at various oxygen concentrations in a microfluidic arrangement. An example application is dose-dependent cellular response can be obtained efficiently at various levels of oxygen tension. Dissolved oxygen (DO), an essential nutrient, is required for energy metabolism of mammalian cells. When cells grow into tumors, dissolved oxygen gradients vary spatially. Similar situation arises in tissues and muscles that are not well vascularized. Since DO has significant effects on cellular physiology, there is a need for a measurement and experimental platform that will enable detailed characterization of cellular responses under various DO levels and DO gradients. To address this, simultaneous sensing of hypoxic perturbation and measuring cellular response in a single semi-automated platform is proposed. The integrated multi-sensing platform is capable of imposing linear oxygen gradients without requiring a continuous supply of oxygen. Monitoring real-time dissolved oxygen tension via integrated thin-film PdO-EPK oxygen sensor, monitoring continuously cell-cycle progression, as well as analyzing dose response effects of multiple drugs on cell proliferation and survival will be accomplished. Two cell types MDA-MB-231 (breast) and U87MG (glioblastoma) will be used for the model experiments. Scientifically, the proposed approach, if successful, will create a measurement platform for conducting oxygen tension studies. The research project is multidisciplinary and integrates bioengineering, cell biology, microfabrication, computational design and modeling, and will expose a full-time PhD student to an interesting learning opportunity. It is planned to incorporate multidisciplinary training in the STEM field through George Mason University?s (GMU) ?Aspiring Scientists Summer Internship Program? designed for promoting research and education in high schools and undergraduate students in the Northern Virginia. Through proposed research on sensor platform the PIs will promote underrepresented minorities in STEM through the ?Louis Stokes Alliance for Minority Participation? program at GMU.