INTRACELLULAR ELECTROCHEMISTRY WITH CARBON NANOTUBE-BASED SENSORS

PI：Schrlau，Michael计划编号：1604893该项目建议创建一种非常微小的生物传感器，用于识别和定量单个活细胞内的生物分子。研究人员提出了一种在活细胞内进行选择性测量的新方法(而不是目前使用的打开细胞的方法)，并在证明该方法后计划实时量化细胞的代谢状态。该项目的目标是创建使用电化学技术在最小扰动下并在较长时间内识别和量化单个活细胞内生物分子的能力。将构建多功能纳米探针，能够注入流体，以便在受限的水微环境中进行独立的电化学测量。然后，利用纳米探针向细胞中注入一种能够进行反应的底物，以选择性地定量衰老相关的β-半乳糖苷酶，这是一种胞浆酶，是许多慢性病的指示物。有人建议使用碳纳米管(CNT)为基础的纳米探针将提供选择性地实时量化细胞衰老的能力。预计在该项目结束时，将有一种新的分析工具和技术来定量单个活细胞中的电活性生物分子。基于模板的简单、可扩展的纳米制造工艺将被用来将纳米电极集成到拉动的玻璃毛细管的尖端，而不需要纳米组装。基于碳纳米管的纳米探针的设计可以很容易地安装标准的细胞生理学仪器，从而便于技术传播、广泛应用和潜在的商业化。这项提议的结果可能会为一种新的微创分析技术提供一流的基于碳纳米管的工具，以收集单个活细胞的定量数据。拟议的努力将产生对基本细胞生理学的新见解，这最终将有助于识别疾病的早期发病，促进药物开发和提高治疗效果。更广泛地说，细胞内电化学的发展将促进纳米技术、材料科学与工程、电化学、细胞生物学和生物医学等科学知识的发展。此外，该项目将为各级学生和新入职人员提供几个高影响力的研究和教育机会，并将支持STEM中代表性不足的群体。

PI: Schrlau, Michael Proposal Number: 1604893This project proposes to create a very tiny biosensor for identifying and quantifying biomolecules inside a single living cell. The investigator proposes a novel way of performing selective measurement inside living cells, (instead of currently used methods of breaking open the cell), and after proving the approach plan is to quantify cellular metabolic state in real time.The goal of the project is to create the ability to identify and quantify biomolecules inside a single living cell with minimal perturbation and over long periods of time using electrochemical techniques. Multifunctional nanoprobe will be constructed, capable of injecting fluids in order to conduct self-contained electrochemical measurements inside confined aqueous microenvironments. The nanoprobe is then utilized to inject a reaction-enabling substrate into the cell for selectively quantifying senescence-associated beta-galactosidase, a cytosolic enzyme, an indicator of many chronic diseases. It is proposed to use carbon nanotube (CNT) -based nanoprobes will provide the ability for selectively quantifying cell senescence in real-time. It is anticipated that at the conclusion of the project, there will be a new analytical tool and technique for quantifying electroactive biomolecules within single living cells. Simple, scalable template-based nanomanufacturing processes will be employed to integrate the nanoelectrodes into the tip of a pulled glass capillary without nanoassembly. The CNT-based nanoprobe is designed to readily fit standard cell physiology instruments, thus facilitating easy technological dissemination, broad utilization, and potential commercialization. The outcome of this proposal is likely to provide a first-in-class CNT-based tool for a novel minimally invasive analytical technique for gathering quantitative data from single living cells. The proposed efforts will generate new insights into fundamental cell physiology, which will ultimately help identify the early onset of diseases, facilitate drug development and improve therapeutic efficacy. More broadly, the development of intracellular electrochemistry will advance scientific knowledge in nanotechnology, materials science and engineering, electrochemistry, cell biology, and biomedicine. Further, the project will provide several high-impact research and educational opportunities for students at all levels as well as recruits and will support underrepresented groups in STEM.