Capillary Channeled Polymer Structures and Chemistries for High Throughput Measurement of Large Molecules

本项目由美国国家科学基金化学分部化学测量与成像项目资助。R. Kenneth Marcus教授和克莱姆森大学的合作者正在开发和表征毛细管通道聚合物（C-CP）纤维作为合成和大型生物分子快速处理的固定相。这些固定相适用于各种仪器形式，并为混合物的分离提供了优势。研究的目的是以经济的方式产生高效和选择性的分离。这项工作是跨学科的，涉及化学、生物工程、数学和材料科学研究小组之间的合作努力。该项目包括隶属于克莱姆森大学纤维和薄膜高级工程中心的研究人员。也有与联邦实验室的合作。如果成功，该项目可能会在南卡罗来纳州的纺织和生物制药行业带来新的商业风险。大分子分离能力的增强对许多商业领域都有影响，包括迅速扩大的蛋白质治疗领域。一种双管齐下的方法被用来更好地影响使用C-CP纤维相的大分子分离。第一个领域的重点是新的实现纤维作为固定相的二维全面的二维液相色谱分离。根据影响从第一维获得的时间“切割”的高质量分离所需的循环时间，评估各种表面化学物质的C-CP相。第二个领域侧重于修饰表面化学物质以影响高水平的选择性。有四类反应：进一步利用脂系配体作为一般反应方法修饰疏水表面，聚酯基纤维的高密度胺化，微波能量影响配体与尼龙6c - cp材料的共价偶联，最后高分子配体在聚丙烯表面吸附进行类特异性分离。这些化学物质用于开发独立的色谱柱（在分析或制备尺度上）以及在多维应用中用作二维的色谱柱。

This project is funded by the Chemical Measurement and Imaging Program of the Chemistry Division at the National Science Foundation. Professor R. Kenneth Marcus and collaborators at Clemson University are developing and characterizing capillary-channeled polymer (C-CP) fibers as stationary phases for rapid processing of synthetic and large biological molecules. These stationary phases are amenable to a wide range of instrumentation formats and offer advantages for the separation of mixtures. The goal of the studies is to produce efficient and selective separations in an economical manner. This work is interdisciplinary, involving collaborative efforts between research groups in chemistry, bioengineering, mathematical and materials sciences. The project includes investigators affiliated with the Clemson University Center for Advanced Engineering of Fibers and Films. There are also collaborations with federal laboratories. If successful, the project may result in new commercial ventures in the textile and biopharmaceutical industries in South Carolina. Enhanced capabilities for large molecule separations have implications across many commercial sectors, including the rapidly expanding area of protein therapeutics. A two-pronged approach is used to better affect macromolecule separations using the C-CP fiber phases. The first area focuses on the novel implementation of the fibers as the stationary phases for dimension two of comprehensive two-dimensional liquid chromatographic separations. C-CP phases of various surface chemistries are evaluated in terms of the cycling times required to affect high quality separations of temporal "cuts" obtained from the first dimension. The second area focuses on modification of surface chemistries to affect high levels of selectivity. There are four classes of reactions: further use of lipid tethered ligands as a general reaction methodology to modify hydrophobic surfaces, high density amination of polyester base fibers, microwave energy to affect covalent coupling of ligands to nylon 6 C-CP materials, and finally the adsorption of macromolecule ligands on polypropylene surface for class-specific separation. These chemistries are used to develop stand-alone columns (on either the analytical or preparative scales) as well as columns for use as dimension two in multidimensional applications.