SBIR Phase I: Development of a Large-scale Process for Production of Vault-Stabilized Enzymes for Industrial Uses and Environmental Cleanup

这个小企业创新研究第一阶段项目的更广泛的影响/商业潜力是开发一个大规模的生产拱顶稳定酶的过程。该项目将开发一种新的基于纳米颗粒的技术，用于稳定酶，使其可用于工业应用和水处理等环境清洁。通过采用可扩展、高产、低成本的拱顶酶生产系统，该公司将能够将各种新产品部署到该领域，用于处理饮用水、地下水、废水和再生水中的某些环境污染物。这将为环境工程师和监管机构提供新的工具，以清洁和保护国家自然资源和公共健康。第一阶段研究项目的技术目标是开发一种基于酵母的纳米级拱顶颗粒生产系统。拱顶纳米颗粒是稳定和增强酶催化工业应用和生物转化性能的有前途的候选者。将酶包装到拱顶颗粒中将酶纯化和固定化结合到单个制造步骤中，并且是用于固定化和保护酶的具有成本效益的环境友好的方法。证明该系统的可行性将允许将拱顶酶简单地集成到工业制造系统中，并且将允许拱顶酶原型被快速放大并部署用于特定的环境修复目标。

The broader impact/commercial potential of this Small Business Innovation Research Phase I project is to develop a large-scale process for production of vault-stabilized enzymes. This project will develop a new nanoparticle-based technology for stabilizing enzymes so that they can be used for industrial applications and environmental cleanup such as water treatment. By employing a scalable, high yield, low cost manufacturing system for vault-enzymes, the company would be able to deploy a variety of new products into the field for treating certain environmental pollutants in drinking water, groundwater, wastewater, and reclaimed water. This would give environmental engineers and regulatory agencies new tools to clean and protect national natural resources and public health.The technical objectives in this Phase I research project are to develop a yeast-based production system for nano-scale vault particles. Vault nanoparticles are promising candidates to stabilize and enhance the performance of enzymes for catalytic industrial applications and biotransformation. The packaging of enzymes into vault particles combines enzyme purification and immobilization into a single manufacturing step, and is a cost-effective, environmentally friendly approach for immobilizing and protecting enzymes. Demonstrating feasibility of this system will allow simple integration of vault-enzymes into industrial manufacturing systems and would allow vault enzyme prototypes to be rapidly scaled up and deployed for specific environmental remediation targets.