Dissecting KDEL receptor clustering at the mammalian cell surface in response tocargo binding, internalization and compartmental transport (A06)

受体簇形成是原核和真核细胞中普遍存在的现象，用于物理感知货物/配体相互作用，并随后将外源信号转化为细胞反应。由于对这些过程背后的分子原理的机制理解仍然缺乏，本项目的主要目标是将遗传、生化和成像数据与理论建模结合起来，解剖潜在的分子过程，并确定参与酵母和哺乳动物细胞中货物诱导的kdel受体集群形成、内化和从质膜到质膜的运输的细胞成分。

Receptor cluster formation is a ubiquitous phenomenon in pro- and eukaryotic cells to physically sense cargo/ligand interactions and subsequently translate an exogenous signal into a cellular response. As a mechanistic understanding of the molecular principles underlying these processes is still lacking, the major goal of this project is to combine genetic, biochemical and imaging data with theoretical modelling to dissect the underlying molecular processes and to identify cellular components involved in cargo-induced KDEL-receptor cluster formation, internalization and transport from and to the plasma membrane in yeast and mammalian cells.