Enhancing Research and Curriculum at California State University Dominguez Hills Through the Acquisition of Next-Generation Sequencing Instrumentation

美国加州州立大学Dominguez Hills （CSUDH）获得Illumina MiSeq下一代测序仪。这台仪器的加入将加强这所为西班牙裔和少数族裔服务的机构的研究和教学。随着CSUDH加大对前沿研究的投入，MiSeq将成为生物科学基础设施和能力建设计划的基石。主要用户将使用MiSeq来研究包括细菌进化、环境微生物学、发育生物学和植物进化遗传学在内的主题。主要是低收入、西班牙裔和非裔美国本科生和研究生，他们在教师的实验室进行研究，将使用当前行业标准的MiSeq技术来回答这些广泛的生物学问题。这些教师和其他教师将把使用MiSeq的真实研究经验融入到他们所教授的课程中，每年可能惠及数百名学生。此外，加州大学圣地亚哥分校的教师将与当地高度贫困的K-12学区和社区学院的教师合作，将MiSeq和基因组数据纳入他们的课程，为生物学职业建立一座桥梁。在这些机构中，教师和学生之间越来越多的互动将加强教育渠道，并帮助这些得不到充分教育的学生设想成为科学家的成功职业。通过收购MiSeq， CSUDH在生物科学领域的新研究驱动型研究人员团队的校园能力将得到极大提高。最初，三个研究小组和五个研究项目将使用下一代测序技术。MiSeq有潜力帮助学院研究人员在以下领域做出贡献：描述环境和基因型对微生物进化的影响，确定花蜜微生物群在传粉者偏好中的作用，确定斑马鱼神经元发育和功能的遗传和微生物因素，以及预测气候变化对植物进化的影响。该设备将极大地提高教师和学生研究人员的研究效率，使研究人员能够轻松、经济地使用诸如宏基因组测序、细菌全基因组重测序、转录组学、靶向测序和基因分型测序等技术。此外，该仪器的加入将有助于将该机构推向更高层次的研究机构，为教师和学生增加机会。

An award is made to California State University Dominguez Hills (CSUDH) to acquire an Illumina MiSeq next-generation sequencer. The addition of this instrument to this campus will enhance research and teaching at the Hispanic-serving, Minority-serving institution. With CSUDH's increased commitment to cutting-edge research, the MiSeq will be the cornerstone of a plan to build infrastructure and capacity in the biosciences. The primary users will employ the MiSeq to study topics including bacterial evolution, environmental microbiology, developmental biology, and plant evolutionary genetics. The primarily low-income, Hispanic and African American undergraduate and graduate students who perform research in the faculty members' labs will use the current industry-standard MiSeq technology to answer these broad biological questions. These and other faculty members will incorporate authentic research experiences using the MiSeq into the courses they teach, potentially reaching hundreds of students per year. Additionally, CSUDH faculty will work with faculty from the local high-poverty K-12 school district and community colleges to include the MiSeq and genomic data in their curricula to build a bridge to biology careers. The increased interaction of faculty and students across these institutions will strengthen the educational pipeline and help these underserved students visualize successful careers as scientists.The on-campus capabilities of CSUDH's team of new, research-driven investigators in the biosciences will be dramatically enhanced by the acquisition of a MiSeq. Initially, three research groups and five research projects will use the next generation sequencing technology. The MiSeq has the potential to assist faculty researchers in making contributions in the following areas: characterizing the effects of environment and genotype on microbial evolution, determining the role the nectar microbiome plays in pollinator preferences, identifying both the genetic and microbial factors that contribute to neuron development and function in zebrafish, and predicting the effects of climate change on plant evolution. This equipment will greatly increase the research productivity of faculty and student researchers, allowing the researchers to easily and cost-effectively use techniques such as metagenomic sequencing, bacterial whole genome re-sequencing, transcriptomics, targeted sequencing, and genotyping-by-sequencing. Further, the addition of this instrument to CSUDH facilities will help catapult the institution into a higher tier of research institutions, increasing opportunities for faculty and students.