Bacillus Genetic Stock Center Emergency Funding

芽孢杆菌在微生物学研究中起着至关重要的作用。这群广泛和多样的细菌合成抗生素的大阵,精细化工、维生素、和工业酶。芽孢杆菌是生物燃料和环保型农药的生产平台。有些物种可作为益生菌或用于疫苗生产；其他国家在医疗保健、食品安全和预防生物恐怖主义方面发挥着重要作用。芽孢杆菌的研究提供了对细菌细胞的生理、发育和行为的深刻见解。芽孢杆菌遗传库存中心（BGSC）保持一个有用的芽孢杆菌菌株的集中收集。每年，BGSC菌株被用于全国数百个创新项目，产生同行评审的出版物和美国专利，本科教学实验室经验和高中科学博览会项目。NSF的支持将加强国家的科学基础设施，确保美国的科学研究和教育界仍然可以获得这些资源。BGSC将收集，维护，记录和分发革兰氏阳性，芽孢内形成细菌，不仅来自芽孢杆菌，还来自许多密切相关的属。馆藏的主干是枯草芽孢杆菌，一种模式生物和生物技术的主力。包括两个完整的敲除文库，涵盖生物体中所有非必需基因，第三个“敲除”文库涵盖必需基因。各种各样的基因工具调节基因表达和构建新的菌株也储备。该收藏还包括大量的生物农药苏云金芽孢杆菌和球形赖生芽孢杆菌、嗜热菌地杆菌、食物腐败菌蜡样芽孢杆菌、促进植物生长的芽孢杆菌和其他重要物种。培养物将被收集并低温储存在俄亥俄州立大学的一个安全地点，并提供给合格的科学家和教育工作者。菌株数据将在BGSC网站（http://bgsc.org）上公开，并整合到美国和欧洲的芽孢杆菌基因和基因组数据库中。

Bacillus plays a vital role in microbiology research. This widespread and diverse group of bacteria synthesizes a large array of antibiotics, fine chemicals, vitamins, and industrial enzymes. Bacillus bacteria serve as production platforms for biofuels and ecofriendly pesticides. Some species are useful as probiotics or in vaccine production; others play important roles in health care, food safety, and bioterrorism prevention. Bacillus research provides deep insights into the physiology, development, and behavior of bacterial cells. The Bacillus Genetic Stock Center (BGSC) maintains a centralized collection for useful Bacillus strains. Each year, BGSC strains are used for hundreds of innovative projects nationwide, resulting in peer-reviewed publications and US patents, undergraduate teaching lab experiences, and high school science fair projects. NSF support will strengthen the nation's scientific infrastructure by ensuring that this resource remains available to America's scientific research and education communities.The BGSC will collect, maintain, document, and distribute Gram-positive, endospore-forming bacteria, not only from Bacillus but from many closely related genera. The backbone of the collection is devoted to B. subtilis, a model organism and biotechnology workhorse. Included are two complete knockout libraries covering every non-essential gene in the organism and a third "knock-down" library covering the essential genes. A wide variety of genetic tools for modulating gene expression and for constructing new strains are also stocked. The collection is rounded out with sizable holdings of the biopesticides B. thuringiensis and Lysinibacillus sphaericus, the thermophile Geobacillus, the food spoilage organism B. cereus, the plant growth-promoting Paenibacillus, and other important species. Cultures will be collected and stored cryogenically at a secure site at the Ohio State University and made available to qualified scientists and educators. Strain data will be made publicly available on the BGSC website (http://bgsc.org) and integrated into Bacillus gene and genome databases in the USA and Europe.