CSBR: Living Stocks: Maintenance of the Bacillus Genetic Stock Center, a Key Resource for Scientific Research and Education

CSBR：活畜：芽孢杆菌遗传库存中心的维护，科学研究和教育的关键资源

• 批准号: 1756219
• 负责人: Tina Henkin
• 金额: $ 49.94万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-12-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1756219&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Maintenance; Bacillus

An award is made to The Ohio State University (OSU) to support the Bacillus Genetic Stock Center (BGSC). Bacillus plays a vital role in microbiology research. This widespread and diverse group of bacteria synthesizes a large array of antibiotics, fine chemicals, vitamins, and industrial enzymes. Bacillus bacteria serve as production platforms for biofuels and ecofriendly pesticides. Some species are useful as probiotics or in vaccine production; others play important roles in sterility assurance, food safety, and bioterrorism prevention. Bacillus research provides insights into bacterial physiology, development, and behavior. The BGSC maintains a centralized collection of useful Bacillus strains. Each year, BGSC strains are used for hundreds of innovative projects nationwide, resulting in peer-reviewed publications and US patents, undergraduate teaching lab experiences, and high school science fair projects. This award will strengthen the nation's scientific infrastructure by ensuring that this resource remains available to America's scientific research and education communities.The BGSC will accession, maintain, document, and distribute Gram-positive, endospore-forming bacteria, not only from Bacillus but from many closely related genera. The backbone of the collection is devoted to B. subtilis, a model organism and biotechnology workhorse. Included are two complete knockout libraries covering every non-essential gene in the organism and a third "knock-down" library covering the essential genes. A fourth library consists of B. subtilis strains that produce membrane proteins marked with fluorescent tags for easy microscopic observation. A wide variety of genetic tools for modulating gene expression and for constructing new strains are also stocked. The collection is rounded out with sizable holdings of the biopesticides B. thuringiensis and Lysinibacillus sphaericus, the thermophile Geobacillus, the food spoilage organism B. cereus, the plant growth-promoting Paenibacillus, and other important species. A small collection of bacteriophages, viruses that infect Bacillus cells, is also maintained. Cultures will be collected and stored cryogenically at a secure site at the OSU. Strain data will be made publicly available on the BGSC website (http://bgsc.org) and integrated into Bacillus gene and genome databases in the USA and Europe. Standardized cultures are provided at a reasonable cost to qualified scientists and educators.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予俄亥俄州州立大学（OSU）以支持芽孢杆菌遗传储备中心（BGSC）。 芽孢杆菌在微生物学研究中起着至关重要的作用。 这种广泛而多样的细菌群合成了大量的抗生素，精细化学品，维生素和工业酶。 芽孢杆菌是生物燃料和生态友好型农药的生产平台。 一些物种可用作益生菌或疫苗生产;其他物种在无菌保证，食品安全和生物恐怖主义预防方面发挥重要作用。 芽孢杆菌的研究提供了深入了解细菌生理学，发育和行为。 BGSC集中收集有用的芽孢杆菌菌株。 每年，BGSC菌株被用于全国数百个创新项目，导致同行评审的出版物和美国专利，本科教学实验室经验和高中科学博览会项目。 该奖项将加强国家的科学基础设施，确保这一资源仍然提供给美国的科学研究和教育界。BGSC将加入，维护，文件，并分发革兰氏阳性，内孢子形成细菌，不仅从芽孢杆菌，但从许多密切相关的属。 该系列的主干是专门为B。枯草杆菌，一种模式生物和生物技术的主力。 包括两个完整的敲除文库，涵盖生物体中的每个非必需基因，以及第三个“敲除”文库，涵盖必需基因。 第四个文库由B组成。枯草杆菌菌株产生标记有荧光标签的膜蛋白，以便于显微镜观察。 还储存了用于调节基因表达和用于构建新菌株的多种遗传工具。 该系列以大量的生物农药B而圆满。苏云金杆菌和球形赖氨酸芽孢杆菌、嗜热土芽孢杆菌、食品腐败菌B。蜡状芽孢杆菌、植物生长促进类芽孢杆菌和其他重要物种。 还保存了少量噬菌体，即感染芽孢杆菌细胞的病毒。 将采集培养物并在OSU的安全地点低温储存。 菌株数据将在BGSC网站（http：//www.example.com）上公开，并整合到美国和欧洲的芽孢杆菌基因和基因组数据库中。bgsc.org 标准化培养物以合理的成本提供给合格的科学家和教育工作者。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Promotion of Bacillus subtilis subsp. inaquosorum, Bacillus subtilis subsp. spizizenii and Bacillus subtilis subsp. stercoris to species status

• DOI: 10.1007/s10482-019-01354-9
• 发表时间: 2019-11-12
• 期刊: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
• 影响因子: 2.6
• 作者: Dunlap, Christopher A.;Bowman, Michael J.;Zeigler, Daniel R.
• 通讯作者: Zeigler, Daniel R.