SBIR Phase I: A Platform for Label-Free, Numerous-Marker-Based Isolation of Functionally Preserved Single Cancer Stem Cells

该SBIR I期项目的更广泛的影响/商业潜力利用了具有多标记物筛选的节点孔感测，用于分离膀胱干细胞的单个尿路上皮癌的活的、功能保留的分离。该工具将有助于更全面的生物标志物发现，并促进靶向治疗的发展。最终，该解决方案将允许临床、学术和工业研究人员从脆弱的原发性膀胱活检亚群中选择性地分离单细胞，用于下游分析和长期的个性化治疗开发。筛选众多参数并以步行可用、可访问和低成本的方式分离膀胱干细胞的靶尿路上皮癌的能力将使下游功能研究成为可能，这将有助于改善患者的预后。结合这里的商业机会，预计将对研究成果和出版物产生重大的积极影响，这将通过将低成本的膀胱干细胞分离仪器引入研究，工业/生物制药，和临床设施。该SBIR第一阶段项目涉及多参数方法，利用无标记的节点-孔隙传感和低-剪切微流体用于活的单细胞分选和分配，同时保留下游测定的活力和功能特性。膀胱癌干细胞的单个活的功能性保存的尿路上皮癌的表征和分离对于确定用于早期癌症检测的普遍接受的临床上准确的生物标志物以及优化的靶向治疗开发是关键的。该项目旨在提供对这些细胞的轻松访问，而无需大量的实验设计和调整，使未经培训的非专家用户能够操作。使用新的检测和分离方法，该平台可用于揭示掩蔽的异质性并实现下游功能研究（如生长、侵袭和化疗抗性）对单独分离的，有活力的，在功能上，该奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的知识产权评估的支持。优点和更广泛的影响审查标准。

The broader impact/commercial potential of this SBIR Phase I project leverages node-pore sensing with multi-marker screening for viable, functionally-preserved isolation of single urothelial carcinoma of the bladder stem cells. This tool will help enable more comprehensive biomarker discovery and facilitate the development of targeted therapies. Ultimately, this solution will allow for clinical, academic, and industrial researchers to selectively isolate single cells from fragile primary bladder biopsy subpopulations for downstream analysis and personalized treatment development in the longer term. The ability to screen for numerous parameters and isolate target urothelial carcinoma of the bladder stem cells in a walk-up usable, accessible, and low-cost manner will enable downstream functional studies that will help lead to improved patient outcomes. In conjunction with the business opportunity here, there is projected to be a significant positive impact on research output and publications that will be enabled by the introduction of a low-cost urothelial carcinoma of the bladder stem cell isolation instrument to research, industrial/biopharma, and clinical facilities.This SBIR Phase I project involves a multi-parametric approach leveraging label-free node-pore sensing and low-shear microfluidics for viable single-cell sorting and dispensing while preserving viability and functional characteristics for downstream assays. Characterization and isolation of single, live functionally-preserved urothelial carcinoma of the bladder cancer stem cells is pivotal for the determination of universally accepted, clinically-accurate biomarkers for early cancer detection as well as optimized targeted therapy development. This project is aimed at delivering easy access to these cells without requiring extensive experimental design and tuning, enabling operation by untrained, non-expert users. Using the novel detection and isolation methodologies, the platform can be used to unveil masked heterogeneity and enable downstream functional studies (such as growth, invasion, and chemotherapeutic resistance) on individually isolated, viable, and functionally-preserved isolated urothelial carcinoma of the bladder stem cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.