Collaborative Research: SG: Phylogenomics and diversification of the snapping shrimp genus Alpheus

捕虾属的捕虾，以其壮观的颜色图案和由其主要爪子产生的爆炸性的捕捉而闻名，是热带珊瑚礁中最多样化和最丰富的动物群体之一。咬虾与珊瑚和鱼类等多种伙伴形成互利关系，并正在成为了解海洋环境中共生和物种多样化的模式群体。然而，这样的研究需要更全面地了解世界上有多少不同的Alpheus物种，以及这些物种之间的关系。这项研究将通过使用下一代DNA测序数据构建该组的进化树来解决这些问题。这些数据将用于测试宿主共生或颜色模式等因素是否推动了这一群体的惊人多样化。研究这一群体的生物多样性将有助于更广泛地了解浅海热带生态系统生物多样性的起源和维持。将通过《生命百科全书》建立物种网页，用于公众宣传和教育目的，并与科学博物馆合作，为本科生举办科学传播讲习班。一名研究生和多名本科生也将接受遗传条形码，物种界定，物种描述和比较进化分析的培训。 这项研究将产生第一个全面的全球性的虾属Alpheus，跨越50%的300+描述的物种，并使用锚定杂交富集产生的基因组数据。这将被用来测试Alpheus长期存在的分类学和形态学问题，如单系的形态定义的物种群和栖息地的使用在驱动的形态收敛的情况下的作用。对野外和博物馆标本进行全面的遗传条形码调查将有助于估计该群体现有的全球生物多样性。通过系统发育重建、实地调查和颜色模式分析，研究人员将测试颜色模式--长期以来被认为具有系统重要性--是否是建立物种边界的重要因素。最后，我们将使用基因组树和全球物种多样性模式的数据来测试Alpheus的物种多样性是否与共生等生态因素有关。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Snapping shrimp in the genus Alpheus, known for their spectacular color patterns and the explosive snap created by their major claw, are one of the most diverse and abundant animal groups in tropical coral reefs. Snapping shrimp form mutualistic relationships with a diversity of partners such as corals and fish and are emerging as a model group for understanding symbiosis and species diversification in marine environments. However, such studies require a more complete understanding of how many different species of Alpheus exist worldwide, and the relationships among these species. This research will address these questions by building an evolutionary tree of the group with next-generation DNA sequencing data. These data will be used to test whether factors such as host symbioses or color patterns drove the spectacular diversification of this group. Studying the biodiversity of this group will help to understand more broadly the origins and maintenance of biodiversity in shallow marine tropical ecosystems. Species pages will be created through the Encyclopedia of Life for public outreach and educational purposes, and a partnership with a science museum will develop workshops in science communication for undergraduates. A graduate student, and multiple undergraduates will also be trained in genetic barcoding, species delimitation, species descriptions, and comparative evolutionary analyses. This research will produce the first comprehensive global phylogeny of snapping shrimp in the genus Alpheus, spanning 50% of the 300+ described species and using phylogenomic data generated via anchored hybrid enrichment. This phylogeny will be used to test long-standing taxonomic and morphological questions in Alpheus, such as the monophyly of morphologically defined species groups and the role of habitat use in driving instances of morphological convergence. A comprehensive genetic barcoding survey of field and museum specimens will help estimate the existing worldwide biodiversity of the group. Using phylogenetic reconstructions, fieldwork, and color pattern analyses, researchers will test whether color patterns - long hypothesized to have systematic importance - are important factors in establishing species boundaries. Finally, the phylogenomic tree and data on worldwide patterns of species diversity will be used to test whether species diversification in Alpheus is linked to ecological factors such as symbiosis.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.