Investigate the roles of endogenous salicylic acids in de novo organogenesis

植物具有独特而重要的能力，可以从一小块组织中再生出新的器官，甚至整个幼苗。这种组织再生的过程是现代农业和园艺的基础。许多水果和蔬菜的繁殖在很大程度上依赖于组织再生技术，如嫁接、组织培养和茎插条生根。这些技术提供了一种非转基因方法来产生嵌合体或改良品种，并最终提高植物的活力和对病原体的抵抗力。因此，了解组织再生的潜在机制对食品质量和安全非常重要。这项研究调查了植物再生是如何在微生物病原体的影响下发生的，微生物病原体一直威胁着我们的农业。新的分子和细胞技术将被用来检测植物激素在调节再生和防御病原体的平衡方面的功能。从这项研究中获得的知识将促进实用和可扩展的策略，以帮助伤口愈合和切割后再生仍然是繁殖的关键瓶颈的物种的再生。拟议的项目还将为不同水平的学生提供现代植物生物技术的体验学习。本研究旨在阐明水杨酸(SA)在创伤诱导新生根再生(DNRR)中的作用。新器官发生是一个引人入胜的发育过程，涉及细胞命运的剧烈转变。调控生物胁迫和再生的途径之间的分子串扰代表了一个重大的知识鸿沟。一个普遍的假设是，NPR家族成员之间的拮抗相互作用微调了SA介导的DNRR抑制。将实现以下具体目标。目的：确定DNRR过程中SA反应和蓄积的时空模式。目的：验证NPR3/4和NPR5/6拮抗调节DNRR的假说。目的：研究NPR4-TGA复合体在DNRR中的功能。这项拟议研究的完成将建立关于内源性SA如何抑制DNRR期间细胞命运转变的基础知识。确定NPR家族成员的贡献及其与TGA转录因子的相互作用将扩大我们对NPR蛋白在SA介导的防御和发育中的不同和重叠功能的认识。对于学生来说，植物再生是一个有趣的发展过程，让他们观察并获得实践经验。参与本科生和K-12教育的精选合作伙伴将参与这项研究，以加强植物发展的体验式学习，特别是针对STEM教育中代表性不足的群体。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

Plants have the unique and important ability to regenerate a new organ or even an entire seedling from a small piece of tissue. This process of tissue regeneration is a foundation for modern agriculture and horticulture. The propagation of many fruits and vegetables heavily relies on techniques involving tissue regeneration, such as grafting, tissue culture, and rooting from stem cuttings. These techniques provide a non-transgenic approach to produce chimera or improved cultivars and, eventually, to increase plants' vigor and resistance to pathogens. Thus, understanding the underlying mechanisms of tissue regeneration is important for food quality and security. This study investigates how plant regeneration occurs under the influence of microbial pathogens, which are constant threats to our agriculture. New molecular and cellular technologies will be used to examine the function of plant hormones in regulating the balance of regeneration and defense against pathogens. Knowledge obtained from this study will promote practical and scalable strategies to help regeneration in species where wound-healing and regeneration after cutting remains a critical bottleneck for propagation. The proposed project will also provide students at different levels with experiential learning of modern plant biotechnology. This study aims to elucidate the role of salicylic acid (SA) in wound-induced de novo Root Regeneration (DNRR), in which adventitious roots form on detached leaves. De novo organogenesis is a fascinating developmental process involving a drastic transition of cell fate. The molecular crosstalk between pathways governing biotic stress and regeneration represents a major knowledge gap. A general hypothesis is that antagonistic interaction between NPR family members fine-tunes the SA-mediated suppression of DNRR. The following specific aims will be addressed. Aim1: Determine the spatiotemporal patterns of SA response and accumulation during DNRR. Aim2: Test the hypothesis that NPR3/4 and NPR5/6 antagonistically regulate DNRR. Aim3: Characterize the function of an NPR4-TGA complex in DNRR. Completion of the proposed study will establish foundational knowledge about how endogenous SA suppresses the cell fate transition during DNRR. Characterizing the contribution of NPR family members and their interactions with TGA transcription factors will expand our knowledge of the distinct and overlapping function of NPR proteins in SA- mediated defense and development. Plant regeneration is an intriguing developmental process for students to observe and to gain hands-on experience. Select partners involved in undergraduate and K-12 education will participate in this study to enhance the experiential learning of plant development, especially for underrepresented groups in STEM education.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.