Collaborative Research: Interactions of Airborne Engineered Nanoparticles with Lung Surfactant Films

纳米技术的快速发展导致了各种类型的工程纳米颗粒的生产和使用。空气中的纳米颗粒不可避免地会释放到环境中，并可能对人类健康造成潜在的后果，特别是一旦吸入，可能会在呼吸道包括肺(肺泡区)造成潜在后果。肺表面活性物质作为一层薄薄的液膜覆盖在肺泡上，代表了在气液界面上抵御这种空气中纳米颗粒的第一道防线。这一合作项目结合了实验和计算研究，旨在研究工程纳米颗粒的物理化学和结构特性对界面流动行为和表面活性物质膜稳定性的影响。这项研究活动还旨在更好地从根本上了解在现实模拟的生理条件下，表面活性剂薄膜与潜在危险的工程纳米颗粒之间的分子相互作用。因此，通过该项目获得的基础知识有望为随后吸入纳米颗粒的保留、转移和清除以及与整个工程纳米颗粒毒性相关的顺序过程提供新的见解。该项目的成果还将促进关于生物纳米粒子在肺部的命运的基本知识，例如冠状病毒病毒粒子，这可能在医学上具有实际意义。该项目的教育和指导方面包括培训研究生先进的表面科学工具和计算技术，指导未被充分代表的本科生进行研究，以及开发与生物系统中纳米颗粒相互作用相关的教学模块和学科。随着工程纳米颗粒的生产和使用日益增加，这些纳米颗粒将不可避免地释放到环境中。因此，工程纳米颗粒在环境中的发生和去向以及对人类健康的潜在后果日益被认为是至关重要的问题。特别是，空气中的纳米颗粒可以导致更大的可能性和程度，从而接触到环境和生物。这一合作项目将集中于提高我们对工程纳米颗粒和肺表面活性物质薄膜之间在多个长度尺度上的分子相互作用的基本理解。该项目将评估吸入的空气中纳米颗粒在呼吸道中的分布和去向。这项研究项目围绕两个具体目标展开。首先，本项目旨在确定吸入的工程纳米颗粒的物理化学和结构特性对肺表面活性物质膜的粘弹性响应和界面稳定性的影响。其次，该项目将产生关于在生理环境中不存在和存在多个长度尺度的工程纳米颗粒的情况下，肺表面活性物质单层和多层中界面相互作用的分子机制的基本数据。为了实现这些目标，将采用实验和计算方法之间的协同组合。实验进展包括一个特别改装的朗缪尔槽，一个带有耗散功能的石英晶体微天平，以及一个定制的粒子发生器单元，以及一个高度复杂的表面力装置。计算部分基于一种新的粗粒度计算框架，用于研究气-液界面的纳米级界面过程，包括耗散粒子动力学模型，用于预测肺表面活性物质单分子层和双分子层掺杂工程纳米粒子的组成依赖于表面张力、弹性、粘度和稳定性。这些纳米尺度界面现象的协作性实验和计算研究有望提供有关肺表面活性物质薄膜的粘弹性性质以及与之相关的对附着/穿透的工程纳米颗粒影响的呼吸剪切应力的响应的定性和定量信息。此外，首席研究人员将生成有关工程纳米颗粒与肺表面活性物质系统的分子相互作用的系统信息，特别是与肺表面活性物质膜结构完整性相关的粘连和融合行为方面的信息。通过该项目获得的发现将提高对纳米颗粒物质(例如冠状病毒病毒粒子)在其他一般细胞膜上的黏附和转移的机械理解。该项目的教育部分包括通过罗格斯大学和加州大学河滨分校提供的各种项目，培训研究生和指导来自代表性不足群体的本科生。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

Rapid developments in nanotechnology have led to the production and use of various types of engineered nanoparticles. It is inevitable that airborne nanoparticles may be released to the environment and may cause potential consequences for human health, in particular, in the respiratory tract including the lung (alveolar region) once inhaled. The lung surfactant, which covers the alveoli as a thin liquid film, represents the first line of defense against such airborne nanoparticles at the air-liquid interface. This collaborative project, involving a synergistic combination of experimental and computational studies, seeks to study the effects of physicochemical and structural properties of engineered nanoparticles on interfacial flow behaviors and stability of surfactant films. This research activity also is aimed at obtaining a better fundamental understanding of the molecular interactions arising between surfactant films and potentially hazardous engineered nanoparticles in realistically imitated physiological conditions. Fundamental knowledge gained through this project is, therefore, expected to provide new insights into the subsequent retention, translocation, and clearance of inhaled nanoparticles and the sequential processes associated with engineered nanoparticle toxicity overall. The project results will also advance the basic knowledge of the fate of biological nanoparticles, such as coronavirus virions, in the lungs that may have practical implications in medicine. Educational and mentoring aspects of this project include training graduate students in advanced surface science tools and computational techniques, mentoring underrepresented undergraduate students in research, and developing teaching modules and subjects relevant to nanoparticle interactions in biological systems.As the production and use of engineered nanoparticles increases day by day, it is inevitable that these nanoparticles will be released to the environment. Therefore, the occurrence and fate of engineered nanoparticles in the environment, and the potential consequences on human health have been increasingly recognized as issues of critical importance. In particular, airborne nanoparticles can result in a much greater likelihood and extent of exposure to the environment and thus living beings. This collaborative project will focus on improving our fundamental understanding of the molecular interactions between engineered nanoparticles and lung surfactant films at multiple-length scales. The project will evaluate the distribution and fate of inhaled airborne nanoparticles in the respiratory tract. This research project is structured around two specific objectives. First, this project aims to determine the effects of physicochemical and structural properties of inhaled engineered nanoparticles on the viscoelastic responses and interfacial stability of lung surfactant films. Second, the project will generate fundamental data concerning the molecular mechanisms of interfacial interactions in lung surfactant monolayers and multilayers in the absence and presence of engineered nanoparticles at multiple length scales in physiological environments. To achieve these goals, a synergistic combination between experimental and computational approaches will be employed. Experimental advances include a specially modified Langmuir trough, a quartz crystal microbalance with dissipation coupled with a custom-made particle generator unit, and a highly sophisticated surface forces apparatus. The computational component is based on a new coarse-grained computational framework for investigations of nanoscale interfacial processes at air-liquid interfaces, including dissipative particle dynamics models to predict the composition dependent surface tension, elasticity, viscosity, and stability of lung surfactant monolayer and bilayers with doped engineered nanoparticles. These collaborative experimental and computational studies of nanoscale interfacial phenomena are expected to provide qualitative and quantitative information on the viscoelastic properties of lung surfactant films and the attendant response to shear stresses upon breathing affected by adhered/piercing engineered nanoparticles. In addition, the principal investigators will generate systematic information on molecular interactions of engineered nanoparticles with the lung surfactant system, especially in terms of adhesion and fusion behaviors that are related to the structural integrity of lung surfactant films. The findings gained through this project will improve mechanistic understanding of the adhesion and translocation of nanoparticulate matter (e.g., coronavirus virions) across other general cell membranes. Educational components of this project involve training graduate students and mentoring undergraduate students from underrepresented groups in engineering through various programs offered at Rutgers University and the University of California, Riverside.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.