MRI: Acquisition of an HPLC Coupled Mass Spectrometer for proteomics, metabolomics, and small molecule applications to expand and upgrade the SJSU PROTEIN Lab

圣何塞州立大学（SJSU）获得了一项奖励，为SJSU核心蛋白质实验室设施购买了一台新的高效液相色谱-质谱仪，该仪器具有改进的代谢组学、蛋白质组学和高分辨率小分子能力。圣何塞州立大学是联邦政府承认的少数民族服务机构。该仪器将通过扩大SJSU的研究能力，使当前和未来的多学科教师受益。这项建议的更广泛的影响是刺激新的研究活动，加强研究人员之间的合作，以及继续培训本科生和硕士生，并让他们接触到最先进的仪器在课堂和研究环境。将举办讲习班，以增加在研究中使用该仪器的教师人数。化学生物学课程将进行修改，重点放在新的工具上，为在STEM领域代表性不足的学生提供宝贵的研究和学习机会。支持研究的知识价值将（1）表征蔬菜水果中发现的化合物如何在细胞代谢中发挥作用，（2）确定乳腺发育的分子机制，（3）表征能够代谢和使用稀土元素生长的细菌，（4）表征联合治疗抑制铜绿假单胞菌生物膜发育的作用机制，（5）了解细胞pH值如何在细胞过程中发挥作用，包括细胞死亡、分化和肿瘤发生，（6）使用新型p450生物催化平台表征小分子开发。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

An award is made to San José State University (SJSU) to acquire a new HPLC-Mass Spectrometer instrument with improved metabolomics, proteomics and high-resolution small molecule capabilities for the SJSU core PROTEIN lab facility. San Jose State University is a federally recognized minority-serving institution. This instrument will benefit both current and future faculty across multiple disciplines by expanding the research capabilities at SJSU. The broader impacts of this proposal are to stimulate new research activities and strengthen collaboration among research faculty as well as continuing to train undergraduate and masters students and expose them to a state-of-the-art instrumentation in both classroom and research settings. Workshops will be developed to increase the number of faculty that will use the instrument in their research. A course in Chemical Biology will be revamped with a focus on the new instrument to provide valuable research and learning opportunities to students who are underrepresented in STEM fields. The intellectual merit of the supported research will (1) characterize how compounds found in fruits in vegetables play roles in cellular metabolism, (2) identify the molecular mechanisms in mammary gland development, (3) characterize bacteria that are able to metabolize and use rare earth elements for growth, (4) characterize the mechanism of action of combination therapies on inhibiting P. aeruginosa biofilm development, (5) understand how cellular pH plays a role in cellular processes including cell death, differentiation, and tumorigenesis, and (6) characterize small molecule development using a novel p450 biocatalysis platform.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.