B cellular regulation of allergen tolerance during specific immunotherapy of IgE-mediated respiratory allergies

在本项目中，将解决以下假设：I）在特异性免疫疗法（SIT）的过程中，变应原特异性B细胞通过产生变应原阻断抗体分泌调节细胞因子并在鼻粘膜中显示出增加的调节活性来充当变应原耐受性的介体。II）变应原应用的不同模式（皮下与舌下SIT）导致明显不同的免疫改变。III）长期变应原耐受性取决于变应原特异性记忆B细胞应答的诱导，其可以通过变应原加强注射来刺激。

In this project the following hypotheses will be addressed:I) During the course of specific immunotherapy (SIT), allergen-specific B cells serve as mediators of allergen tolerance by - producing allergen-blocking antibodies- secreting regulatory cytokines- and showing increased regulatory activity in the nasal mucosa.II) Different modes of allergen application (subcutaneous vs. sublingual SIT) result in distinctly different immune alterations.III) Long term allergen tolerance depends on the induction of an allergen-specific memory B cell response, which can be stimulated by allergen booster injections.