MRI: Acquisition of a SEC-MALS Instrument for Research and Teaching at California State University, Sacramento

该奖项由主要研究仪器项目和化学研究仪器项目共同支持。加州州立大学萨克拉门托分校(SAC State)正在购买一台体积排阻色谱多角光散射(SEC-MALS)分析仪器，其中将包括一台带有UV检测器和高通量分析自动进样器的高效液相色谱仪器、一台折射率(RI)检测器和一台MALS检测器，以支持凯瑟琳·麦克雷诺兹教授的同事琳达·罗伯茨和斯特凡·保拉的研究。简而言之，SEC-MALS是一种确定溶液中分子摩尔质量和大小的绝对技术。该仪器结合了尺寸排除层析和多角度光散射，提供了一种先进的表征技术，克服了柱校准法的许多限制。SEC-MALS是一种非常有价值和有用的技术，用于表征各种大分子，包括合成聚合物、脂类、蛋白质和其他生物材料。此次收购加强了该大学和该地区的研究基础设施。这一工具加强了许多部门各级学生的教育、研究和教学努力，并提供了在附近机构使用的便利条件。该工具将用于培训以本科生为主的机构的学生人口，该机构也被归类为西班牙裔服务机构和亚裔美国人/美洲原住民/太平洋岛民服务机构，其中很大一部分学生人口被认为来自代表性不足的少数群体。该仪器为学生提供了使用他们随身携带的重要仪器的经验。该仪器的获奖包括尺寸排除多角度光散射(SEC-MALS)，旨在加强各级的研究和教育。它特别影响到包括化学、生物化学和聚合物科学在内的领域。研究的重点是分析合成/生物大分子，并获得有关这些分子的重要结构信息。这项工作包括研究人工合成的糖共聚物与各种病毒刺突蛋白(HIV和SARS-CoV-2)之间的相互作用，以更好地了解早期病毒与宿主细胞的结合。该仪器还将能够研究脂质体作为生前生命形式的模型，使用该仪器来确定脂质体制剂的直径和稠度，以及检查apoA-I的异常寡聚，这有助于在动脉斑块中沉积淀粉样蛋白。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is jointly supported by the Major Research Instrumentation and the Chemistry Research Instrumentation Programs. California State University-Sacramento (Sac State), is acquiring an instrument for size exclusion chromatography with multi- angle light scattering (SEC-MALS) analysis, which will include an HPLC instrument with a UV detector and an autosampler for high throughput analysis, a refractive index (RI) detector, and a MALS detector to support the research of Professor Katherine McReynolds colleagues Linda Roberts and Stefan Paula. In brief, SEC-MALS is an absolute technique for determining molar mass and size of molecules in solution. The instrument involves the combination of size-exclusion chromatography with multi-angle light scattering and offers an advanced characterization technique and overcomes the many limitations of column calibration. SEC-MALS is an extremely valuable and useful technique for the characterization of a wide variety of macromolecules, including synthetic polymers, lipids, proteins, and other biomaterials. The acquisition strengthens the research infrastructure at the University and regional area. This instrument enhances the educational, research, and teaching efforts of students at all levels in many departments as well as provides accessibility for use at nearby institutions. The instrument will be used to trains a student population at the primarily undergraduate institution that is also classified as Hispanic serving and Asian American/Native American/Pacific Islander serving with a large portion of the student population identifying as being from underrepresented minority groups. The instrument gives students experience using vital instrumentation that they carry with them into their careers.The award of this instrument that includes size exclusion chromatography with multi- angle light scattering (SEC-MALS) is aimed at enhancing research and education at all levels. It especially impacts areas including chemistry, biochemistry and polymer science. Research is focused on the analysis of synthetic/biologic macromolecules and derive important structural information about these molecules. This work includes the study of the interactions between synthetic glycopolymers and various viral spike proteins (HIV and SARS-CoV-2) to better understand early-stage viral binding to host cells. The instrument will also enable the study of liposomes as models for prebiotic life forms, using the instrument to determine diameter and consistency of liposome preparations, as well as the examination of the abnormal oligomerization of apoA-I, which contributes to deposition of amyloid protein in arterial plaque.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.