Research Infrastructure: Sustaining Medicago truncatula mutant resources for legume biology research

豆科植物在可持续农业中发挥着至关重要的作用，因为它们能够与土壤细菌共生，为植物生长固定氮气。蒺藜苜蓿是研究豆科植物特有的生物学过程的模式豆科植物，因为大多数豆科植物具有复杂的基因组，使得遗传操作不可行。突变体M.因此，蒺藜是了解豆科植物基因功能的不可替代的资源。从2004年到2013年，PI和Co-PI在M.蒺藜此外，还在M.蒺藜这些突变体资源托管在俄克拉荷马州州立大学，并通过基于网络的数据库向世界各地的用户提供。从2007年到2022年，超过15，500个突变株系已分发给29个国家的220个研究小组。该项目的学生将接受基础设施管理培训，以处理种子申请，种子检索，种子清洗，文件，发票，运输和PCR反向筛选，包括引物设计，PCR扩增，凝胶电泳和测序。在Noble研究所开发了000个蒺藜苜蓿反转录转座子Tnt 1插入系（Tnt 1），在这些系中，约400个，通过使用下一代测序回收000个侧翼序列标签（FST）。此外，还在M中产生了大规模的快中子轰击（FNB）缺失突变体收集。蒺藜2021年，这些资源被转移到俄克拉荷马州州立大学农业生物科学研究所。全世界的用户可以通过基于网络的数据库访问突变体集合，该数据库包含表型描述和单个品系的照片，BLAST可搜索的FST序列和JBrowser基因组查看器。自2007年首次分发种子以来，总共向29个国家的220个研究小组分发了15，100多个Tnt 1系和400个FNB系。突变株系的持续分布和表征有望支持与生物经济或生物技术相关的基本生物机制和应用研究。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Legumes play a crucial role in sustainable agriculture due to their capability of symbiotic association with soil bacteria to fix nitrogen gas for plant growth. Medicago truncatula is a model legume species to study biological processes that are unique to legumes because most legumes have complex genomes that make genetic manipulations infeasible. Mutants in M. truncatula are thus irreplaceable resources in understanding gene functions in legumes. From 2004 to 2013, the PI and the Co-PI generated legume mutant collection with ~21,000 insertional mutant lines in M. truncatula. In addition, a large-scale fast neutron deletion mutant collection was also generated in M. truncatula. These mutant resources are hosted at Oklahoma State University and are accessible to worldwide users through a web-based database. From 2007 to 2022, more than 15,500 mutant lines have been distributed to 220 research groups in 29 countries. Students on the project will receive infrastructure management training to handle seed requests, seed retrieval, seed cleaning, documentation, invoicing, shipping, and PCR reverse screening that includes primer designing, PCR amplification, gel electrophoresis and sequencing.From 2004 to 2013, ~21,000 Medicago truncatula retrotransposon Tnt1 insertion lines (Tnt1) were developed at the Noble Research Institute and out of these lines, about 400,000 flanking sequence tags (FSTs) were recovered by using next generation sequencing. In addition, a large-scale fast neutron bombardment (FNB) deletion mutant collection was also generated in M. truncatula. In 2021, these resources were transferred to the Institute for Agricultural Biosciences, Oklahoma State University. The mutant collections are accessible to users worldwide through a web-based database, which contains phenotype descriptions and photos of individual lines, BLAST-searchable FST sequences and a JBrowser genome viewer. Since the first seed distribution in 2007, a total of more than 15,100 Tnt1 lines and 400 FNB lines have been distributed to 220 research groups in 29 countries. Continued distribution and characterization of mutant lines is expected to support research into basic biological mechanisms and applications related to the bioeconomy or biotechnology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.