PFI-RP: Advanced cryopreservation of complex bioengineered tissues

这一创新伙伴关系-研究伙伴关系（PFI-RP）项目的更广泛影响/商业潜力对商业空间工业和生物医学工业都是深远的。提出的产品解决了地球和近地轨道上复杂三维（3D）组织生物制造的关键技术差距和瓶颈。该技术结合了微重力优化的培养容器和先进的低温保存能力，将对太空生物制造界产生革命性的影响，并为太空制造的生物制品开辟了广泛的地球生物医学应用。这种创新可能会加速建立新的天基生物医学产业，并促使进一步创新，以开发自我维持和完全自动化的生物卫星，为地球健康生产空间制造的生物制品。该项目为附属研究生提供了一个培训机会，以获得实际的生物技术创业经验。拟议的项目是基于两项前沿技术的拟议整合：微重力促进的组织自组装和等弦过冷组织保存。该项目如果成功，将确定热力学和生物学参数，从而在微重力优化的组织培养生物反应器中实现复杂3D生物工程组织的等时程过冷低温保存。目标是开发一种商业化的组织工程系统，该系统具有先进的低温保存能力，可以在近地轨道和地球之间生成、储存和运输活体、复杂的3D生物工程组织。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this Partnerships for Innovation – Research Partnership (PFI-RP) project is far-reaching for both the commercial space industry and the biomedical industry. The proposed product addresses a critical technological gap and bottleneck in complex three-dimensional (3D) tissue biomanufacturing on Earth and in low-Earth orbit. The proposed technology, which combines a microgravity-optimized culture vessel with advanced cryopreservation capabilities, would be transformative for the in-space biomanufacturing community and open a broad range of on-Earth biomedical applications for space-made biologics. The innovation may accelerate the establishment of a new space-based biomedical industry and motivate further innovation to develop self-sustaining and fully automated biosatellites to produce space-made biologics for Earth health benefits. This project represents a training opportunity for the affiliated graduate students to gain hands-on, real-world, biotechnology entrepreneurial experience.The proposed project is based on the proposed integration of two cutting-edge technologies: microgravity-facilitated tissue self-assembly and isochoric supercooling tissue preservation. This project, if successful, will determine the thermodynamic and biological parameters that will enable isochoric supercooling cryopreservation of complex 3D bioengineered tissues within a microgravity-optimized tissue culture bioreactor. The goal is to develop a commercialization-ready tissue engineering system with advanced cryopreservation capabilities that can generate, store, and transport living, complex, 3D bioengineered tissues between low-Earth orbit and Earth.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.