PFI-RP: Advanced cryopreservation of complex bioengineered tissues

这一创新研究伙伴关系项目的广泛影响/商业潜力对商业航天业和生物医学业都具有深远意义。 拟议的产品解决了地球和低地球轨道上复杂三维（3D）组织生物制造的关键技术差距和瓶颈。拟议的技术将微重力优化培养容器与先进的冷冻保存能力相结合，将为太空生物制造界带来变革，并为太空生物制剂开辟广泛的地球生物医学应用。 这一创新可能会加速建立一个新的天基生物医学产业，并推动进一步创新，以开发自我维持和全自动化的生物卫星，生产有利于地球健康的空间生物制剂。该项目是一个培训机会，为附属研究生获得动手，现实世界，生物技术创业经验。拟议的项目是基于两个尖端技术的建议整合：微重力促进组织自组装和等容过冷组织保存。该项目如果成功，将确定热力学和生物学参数，使复杂的3D生物工程组织在微重力优化的组织培养生物反应器内等容过冷冷冻保存。 其目标是开发一种具有先进低温保存能力的商业化组织工程系统，可以在低地球轨道和地球之间生成、储存和运输活的、复杂的3D生物工程组织。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

The broader impact/commercial potential of this Partnerships for Innovation – Research Partnership (PFI-RP) project is far-reaching for both the commercial space industry and the biomedical industry. The proposed product addresses a critical technological gap and bottleneck in complex three-dimensional (3D) tissue biomanufacturing on Earth and in low-Earth orbit. The proposed technology, which combines a microgravity-optimized culture vessel with advanced cryopreservation capabilities, would be transformative for the in-space biomanufacturing community and open a broad range of on-Earth biomedical applications for space-made biologics. The innovation may accelerate the establishment of a new space-based biomedical industry and motivate further innovation to develop self-sustaining and fully automated biosatellites to produce space-made biologics for Earth health benefits. This project represents a training opportunity for the affiliated graduate students to gain hands-on, real-world, biotechnology entrepreneurial experience.The proposed project is based on the proposed integration of two cutting-edge technologies: microgravity-facilitated tissue self-assembly and isochoric supercooling tissue preservation. This project, if successful, will determine the thermodynamic and biological parameters that will enable isochoric supercooling cryopreservation of complex 3D bioengineered tissues within a microgravity-optimized tissue culture bioreactor. The goal is to develop a commercialization-ready tissue engineering system with advanced cryopreservation capabilities that can generate, store, and transport living, complex, 3D bioengineered tissues between low-Earth orbit and Earth.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.