Integrating Data Science and Hands-on Experience into the Community College Biotechnology Classroom with Applications to Antibody Engineering

抗体是脊椎动物免疫系统自然产生的蛋白质。它们在生物技术中很重要，在研究、诊断和治疗中充当试剂。Kapi 'olani社区学院（KCC）的学生将以抗体工程为培训重点，学习生物技术工作人员的实用技能。事实上，生物技术行业正在经历一个增长期，需要经过多种学科培训的人才，包括数据科学、人工智能和机器学习。此外，由于严重依赖旅游业，疫情严重影响了夏威夷的经济。因此，为了使经济多样化，并在高科技、知识型和新兴产业（如生物技术）中创造高薪工作，夏威基州对创新产业的多样化很感兴趣，以确保该州能够在技术先进的世界中发挥作用。作为一所少数族裔服务机构，夏威夷本土学生占20%左右，KCC为提高STEM的多样性、公平性和包容性做出了贡献。将抗体工程引入KCC常规生物技术课程的独特机会将有助于为夏威夷不断发展的生物技术和生物制造业建立一支有能力的劳动力队伍。该项目还将促进夏威夷经济的多样化，并对夏威夷本土学生和夏威夷州乃至全国的生物技术产业产生积极影响。该项目将为Kapi 'olani社区学院的生物技术项目引入新的抗体工程模块。第一个目标是培养学生进入生物技术行业。第二个目标是展示数据科学在生物技术和生物制造中的重要性，并增强本科生的经验。第三个目标是训练学生使用设计-构建-测试范式进行抗体的设计、生产、纯化和表征。实现这些目标将扩展单克隆抗体服务设施和培训中心（MASFTC）的能力。该项目的具体目标是：(1)开发课堂和实验室模块，以支持基于体验的本科学习和研究活动，将设计-构建-测试范式应用于抗体工程。(2)开发基于网络的图形用户界面的数据科学和生物信息学教育模块，以指导课堂活动和实验室组成部分，重点是本科生的研究经历。(3)建立KCC抗体卓越中心（KCC- ace）数据库，记录KCC师生进行的设计-构建-测试活动。该项目由先进技术教育计划资助，该计划侧重于为推动国家经济的先进技术领域培养技术人员。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

Antibodies are proteins naturally produced by the vertebrate immune system. They are important in biotechnology, serving as reagents in research, diagnostics, and therapeutics. Using antibody engineering as a focus for training, students at Kapi’olani Community College (KCC) will learn practical skills for the biotechnology workforce. Indeed, the biotechnology industry is experiencing a period of growth that requires talented personnel trained across many disciplines, including data science, artificial intelligence, and machine learning. Moreover, the pandemic severely impacted the economy in Hawai’i due to its heavy reliance on tourism. Thus, to diversify the economy and create high-paying jobs in high-tech, knowledge-based, and emerging industries such as biotechnology, the state of Hawai’i is interested in diversifying innovative industries to ensure the state can function in a technologically advanced world. As a minority serving institution, with a native Hawaiian student population of ~20%, KCC contributes to the enhancement of diversity, equity, and inclusion in STEM. The unique opportunity to introduce antibody engineering into KCC’s regular biotechnology curriculum will contribute to the building of a capable workforce for the growing biotechnology and biomanufacturing sectors in Hawai’i. This project will also contribute to the diversification of the economy in Hawai’i and have a positive impact on native Hawaiian students and the biotech industry in the state of Hawai’i and across the nation.This project will introduce new antibody engineering modules to the biotechnology program at Kapi’olani Community College. The first goal is to train students to enter the biotechnology workforce. The second goal is to demonstrate the importance of data science in biotechnology and biomanufacturing and to enhance the undergraduate experience. The third goal is to train students in the design, production, purification, and characterization of antibodies using the Design-Build-Test paradigm. Accomplishing these goals will extend the capabilities of the Monoclonal Antibody Service Facility and Training Center (MASFTC). The specific aims for this project are: (1) Develop classroom and laboratory modules to support experiential-based undergraduate learning and research activities using the Design-Build-Test paradigm as applied to antibody engineering. (2) Develop data science and bioinformatics education modules with a web-based graphical user-interface to guide classroom activities and laboratory components with an emphasis on undergraduate research experiences. (3) Establish the KCC-Antibody Center of Excellence (KCC-ACE) database to record the Design-Build-Test activities carried out by KCC students and faculty. This project is funded by the Advanced Technological Education program that focuses on the education of technicians for the advanced-technology fields that drive the nation's economy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.