Spatial and temporal coordination of RNA translation, replication and assembly in a membranous plus-strand RNA virus replication factory (11)

我们在确定正链RNA病毒复制细胞器（RO）生物发生和功能的相关病毒和细胞因子方面取得了重要进展，并揭示了HCV和SARS CoV 2之间令人惊讶的相似之处。此外，我们确定了一个敏感的步骤，在登革热复制，原来是非常适合抗病毒治疗，通过采用一个集成的数学模型在单细胞分辨率。在下一个资助期内，我们希望继续对涉及RO生物发生和功能的病毒和宿主细胞因子进行比较分析。生物信息学和系统生物学的方法将包括捕捉RO形成，病毒复制和病毒粒子组装的动态。

We made important progress in identifying relevant viral and cellular factors in plus-strand RNA virus replica-tion organelle (RO) biogenesis and functionality, and revealed surprising parallels between HCV and SARS CoV 2. Furthermore, we identified a sensitive step in Dengue replication that turned out to be well suited for antiviral therapy by employing an integrated mathematical model at single-cell resolution. In the next funding period, we want to continue conducting comparative analyses of viral and host cell factors involved in RO biogenesis and function across phylogenetically distant viruses. Bioinformatics and systems biology approaches will be included to capture the dynamics of RO formation, viral replication and virion assembly.