Genetic and molecular analysis of epistatic interactions in flowering time pathways identified in a barley multi-parent advanced generation intercross (MAGIC) population

提出这一建议所依据的假说：基于魔术的群体构成了遗传分析的坚实的基础，用于上位性效应的遗传分析和鉴定通过相互补充或增强或通过一个座位对另一个座位的抑制活性进行正向互作的基因；对大麦开花时间具有上位性影响的基因在转录水平上受到时间和组织特异性的调控，还可能是相互作用的；在大麦中获得的数据可以转移到其他重要的和遗传上更复杂的作物，如小麦。基于魔术种群的上位性分析为鉴定从营养到生殖阶段的新基因互作提供了一个平台。在小麦中对大麦结果的验证将为小麦等复杂基因组中的这一特征提供新的证据，并表明数据在谷物物种之间的可传递性。这一结果将有助于更好地理解开花起始的途径，并将为谷物育种者提供基于知识的育种计划工具。这项研究的部分目标是：通过更高密度的遗传图谱(50K SNP芯片)改进单倍型区块构建，从而提高魔术群体中开花时间的上位性效应估计的分辨率；在所选基因座的其他冬春大麦组合小组中估计和验证上位性效应；寻找具有上位性效应的候选基因，并在结构水平上分析等位基因变异；互作基因的动力学和植物器官表达，以更好地了解开花起始上位性的分子基础；将已确定的上位性效应从大麦转移到小麦，并验证其作为一种重要的经济自交作物。

Hypothesis on which the proposal is based on:MAGIC based populations constitute a solid basic for genetic analysis of epistatic effects and identification of genes interacting in positive direction by complementing or enhancing each other or negative direction with suppressor activity of one locus towards the other;Genes with epistatic effects to flowering time are regulated at transcription level in time and tissue specific manner and possibly reciprocally;The data obtained in barley can be transferred to other important and genetically more complex crops like wheat.The main goal is the functional characterization of epistatic effects for flowering time in barley at genetic and molecular level. MAGIC population based epistatic analysis offers a platform for identification of novel gene interactions in the transition from vegetative to reproductive phase. The validation in wheat of the results from barley will give new evidence for the trait in complex genomes like wheat and show the transferability of the data across the cereal species. The results will help for a better understanding of the pathways leading to flowering initiation and will offer the cereal breeders tools for knowledge-based breeding programs. Partial objectives of this study are:Improved haplotype block building through higher dense genetic map (50K SNP chip) resulting in increase of the resolution of estimated epistatic effects for flowering time in the MAGIC populationEstimation and validation in other winter and spring barley association panels of the selected loci; Identification of candidate genes at loci with epistatic effects and analysis of allelic variation at the structural level; Kinetic and plant organ expression of interacting genes to better understand the molecular basis of epistasis in flowering initiation;Transfer and validation of determined epistatic effects from barley to wheat as an economical important self pollinating crop.